Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies

Thulaganyo Domfe, Zikhona Njengele-Tetyana, Nikiwe Mhlanga, Phumlani Tetyana, Amanda Skepu, Jane Catherine Ngila, Lucky Mashudu Sikhwivhilu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Rift Valley fever (RVF) is a mosquito-borne zoonotic disease that is caused by the Rift Valley fever virus (RVFV); Bunyaviridae: Phlebovirus. RVF disease can affect several different species, including ruminants, camels and humans and thus present a dual threat to public health and livestock food production in endemic regions. In livestock, the RVFV infection is characterised by an acute hepatitis, abortion and high mortality rates in new-born animals. The current RVF diagnostic techniques have shown good sensitivity. However, they require extensive sample processing and complex instrumentation. Owing to speed, low cost, ease of use, and most importantly, the ability to diagnose diseases at sites where they are managed, lateral flow immunoassays (LFIA) are the most widely used point-of-care (POC) tools for disease diagnosis. In this study, a lateral flow assay (LFA) device that is able to detect antibodies against RVFV, with a minimum detectable concentration of 0.125 mg/mL, was successfully developed. The LFA also successfully detected RVFV antibodies in reference RVFV sera. Protein A (ProA), which has the ability to bind immunoglobulins from different species, was used in the detection probe, giving the developed RVFV LFA potential for multi-species diagnosis.

Original languageEnglish
Article number2664
Issue number11
Publication statusPublished - Nov 2022


  • Rift Valley fever virus
  • diagnostic assay
  • enzyme-linked immunosorbent assay
  • protein expression

ASJC Scopus subject areas

  • Clinical Biochemistry


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