Development of a microsatellite library for the flightless moth Pringleophaga marioni Viette (Lepidoptera: Tineidae)

C. W. Groenewald; C. Born; S. L. Chown; B. Jansen van Vuuren

doi:10.1007/s12686-010-9344-9

Development of a microsatellite library for the flightless moth Pringleophaga marioni Viette (Lepidoptera: Tineidae)

C. W. Groenewald, C. Born, S. L. Chown, B. Jansen van Vuuren

Stellenbosch University

Research output: Contribution to journal › Comment/debate

4 Citations (Scopus)

Abstract

Fifteen microsatellite markers were isolated from an enriched microsatellite library developed specifically for Pringleophaga marioni, a flightless moth species from the sub-Antarctic Prince Edward Islands. Of these, 12 markers were polymorphic in a population of 44 individuals collected on Marion Island. Between 2 and 7 alleles were amplified per marker and the expected heterozygosities (H _E) for these ranged from 0.046 to 0.753. These microsatellite markers reported here are the first for P. marioni and will be used to investigate conservation genetic aspects of this species on the Prince Edward Islands.

Original language	English
Pages (from-to)	291-294
Number of pages	4
Journal	Conservation Genetics Resources
Volume	3
Issue number	2
DOIs	https://doi.org/10.1007/s12686-010-9344-9
Publication status	Published - Apr 2011
Externally published	Yes

Keywords

Fiasco method
Flightless moth
Marion Island
Microsatellite
Sub-Antarctic

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s12686-010-9344-9

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title = "Development of a microsatellite library for the flightless moth Pringleophaga marioni Viette (Lepidoptera: Tineidae)",

abstract = "Fifteen microsatellite markers were isolated from an enriched microsatellite library developed specifically for Pringleophaga marioni, a flightless moth species from the sub-Antarctic Prince Edward Islands. Of these, 12 markers were polymorphic in a population of 44 individuals collected on Marion Island. Between 2 and 7 alleles were amplified per marker and the expected heterozygosities (H E) for these ranged from 0.046 to 0.753. These microsatellite markers reported here are the first for P. marioni and will be used to investigate conservation genetic aspects of this species on the Prince Edward Islands.",

keywords = "Fiasco method, Flightless moth, Marion Island, Microsatellite, Sub-Antarctic",

author = "Groenewald, {C. W.} and C. Born and Chown, {S. L.} and {Jansen van Vuuren}, B.",

year = "2011",

month = apr,

doi = "10.1007/s12686-010-9344-9",

language = "English",

volume = "3",

pages = "291--294",

journal = "Conservation Genetics Resources",

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T1 - Development of a microsatellite library for the flightless moth Pringleophaga marioni Viette (Lepidoptera: Tineidae)

AU - Groenewald, C. W.

AU - Born, C.

AU - Chown, S. L.

AU - Jansen van Vuuren, B.

PY - 2011/4

Y1 - 2011/4

N2 - Fifteen microsatellite markers were isolated from an enriched microsatellite library developed specifically for Pringleophaga marioni, a flightless moth species from the sub-Antarctic Prince Edward Islands. Of these, 12 markers were polymorphic in a population of 44 individuals collected on Marion Island. Between 2 and 7 alleles were amplified per marker and the expected heterozygosities (H E) for these ranged from 0.046 to 0.753. These microsatellite markers reported here are the first for P. marioni and will be used to investigate conservation genetic aspects of this species on the Prince Edward Islands.

AB - Fifteen microsatellite markers were isolated from an enriched microsatellite library developed specifically for Pringleophaga marioni, a flightless moth species from the sub-Antarctic Prince Edward Islands. Of these, 12 markers were polymorphic in a population of 44 individuals collected on Marion Island. Between 2 and 7 alleles were amplified per marker and the expected heterozygosities (H E) for these ranged from 0.046 to 0.753. These microsatellite markers reported here are the first for P. marioni and will be used to investigate conservation genetic aspects of this species on the Prince Edward Islands.

KW - Fiasco method

KW - Flightless moth

KW - Marion Island

KW - Microsatellite

KW - Sub-Antarctic

UR - http://www.scopus.com/inward/record.url?scp=84859873593&partnerID=8YFLogxK

U2 - 10.1007/s12686-010-9344-9

DO - 10.1007/s12686-010-9344-9

M3 - Comment/debate

AN - SCOPUS:84859873593

SN - 1877-7252

VL - 3

SP - 291

EP - 294

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 2

ER -

Development of a microsatellite library for the flightless moth Pringleophaga marioni Viette (Lepidoptera: Tineidae)

Abstract

Keywords

ASJC Scopus subject areas

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