TY - JOUR
T1 - Development, chemometric-assisted optimization and in-house validation of a modified pressurized hot water extraction methodology for multi-mycotoxins in maize
AU - Gbashi, Sefater
AU - Njobeh, Patrick Berka
AU - De Saeger, Sarah
AU - De Boevre, Marthe
AU - Madala, Ntakadzeni Edwin
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Effective management of mycotoxins rely on stringent regulation and routine surveillance of food/feed commodities via efficient analysis, hence the continuous need for improved methods. The present study developed, optimized and validated a modified pressurized hot water extraction (PHWE) method for the simultaneous extraction of multi-mycotoxins from maize and subsequent quantification on LC-MS/MS. The PHWE system was modified using ethanol (EtOH) as a cosolvent, while a numerical modelling approach, the central composite design (CCD), was adopted for the optimization of the extraction conditions. Using the optimized method, it was possible to effectively extract and quantify 15 different mycotoxins from maize in a single step with satisfactory linearities (0.986–0.999), recoveries (14–124%) and other associated method validation parameters. Further efficacious application of the method to real samples re-affirmed the prospects of PHWE as a suitable, cost-effective and greener alternative to traditional methods of mycotoxin extraction.
AB - Effective management of mycotoxins rely on stringent regulation and routine surveillance of food/feed commodities via efficient analysis, hence the continuous need for improved methods. The present study developed, optimized and validated a modified pressurized hot water extraction (PHWE) method for the simultaneous extraction of multi-mycotoxins from maize and subsequent quantification on LC-MS/MS. The PHWE system was modified using ethanol (EtOH) as a cosolvent, while a numerical modelling approach, the central composite design (CCD), was adopted for the optimization of the extraction conditions. Using the optimized method, it was possible to effectively extract and quantify 15 different mycotoxins from maize in a single step with satisfactory linearities (0.986–0.999), recoveries (14–124%) and other associated method validation parameters. Further efficacious application of the method to real samples re-affirmed the prospects of PHWE as a suitable, cost-effective and greener alternative to traditional methods of mycotoxin extraction.
KW - Central composite design
KW - Chemometric-assisted optimization
KW - Mycotoxins
KW - Pressurized hot water extraction
UR - http://www.scopus.com/inward/record.url?scp=85073628646&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2019.125526
DO - 10.1016/j.foodchem.2019.125526
M3 - Article
C2 - 31648172
AN - SCOPUS:85073628646
SN - 0308-8146
VL - 307
JO - Food Chemistry
JF - Food Chemistry
M1 - 125526
ER -