Development and characterization of 13 new, and cross amplification of 3, polymorphic nuclear microsatellite loci in the common myna (Acridotheres tristis)

Cécile Berthouly-Salazar; Phillip Cassey; Bettine Jansen van Vuuren; Berndt Janse van Rensburg; Cang Hui; Michael G. Gardner; Johannes J. Le Roux

doi:10.1007/s12686-012-9607-8

Development and characterization of 13 new, and cross amplification of 3, polymorphic nuclear microsatellite loci in the common myna (Acridotheres tristis)

Cécile Berthouly-Salazar, Phillip Cassey, Bettine Jansen van Vuuren, Berndt Janse van Rensburg, Cang Hui, Michael G. Gardner, Johannes J. Le Roux

Zoology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We used a next generation sequencing (NGS) approach to screen for genome-wide nuclear microsatellite loci in the common (Indian) myna, Acridotheres tristis. In addition, markers previously developed for other Sturnidae species were tested for cross-amplification in A. tristis. In total, we identified 20 loci from NGS data and tested 26 loci for cross-amplification. Out of all loci (NGS developed and cross-amplified), 16 unlinked loci showed polymorphism, ranging from 2 to 9 alleles per locus. Test individuals were obtained from the invasive ranges of common myna in South Africa. Overall, expected and observed heterozygosities ranged from 0.089 to 0.802 and from 0.094 to 0.906, respectively. These markers will be used to shed light on invasion genetics and landscape-scale dynamics of invasive A. tristis in South Africa.

Original language	English
Pages (from-to)	621-624
Number of pages	4
Journal	Conservation Genetics Resources
Volume	4
Issue number	3
DOIs	https://doi.org/10.1007/s12686-012-9607-8
Publication status	Published - Sept 2012

Keywords

Acridotheres tristis
Common myna
Invasive species
Microsatellite

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12686-012-9607-8

Cite this

Berthouly-Salazar, C., Cassey, P., van Vuuren, B. J., van Rensburg, B. J., Hui, C., Gardner, M. G., & Le Roux, J. J. (2012). Development and characterization of 13 new, and cross amplification of 3, polymorphic nuclear microsatellite loci in the common myna (Acridotheres tristis). Conservation Genetics Resources, 4(3), 621-624. https://doi.org/10.1007/s12686-012-9607-8

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title = "Development and characterization of 13 new, and cross amplification of 3, polymorphic nuclear microsatellite loci in the common myna (Acridotheres tristis)",

abstract = "We used a next generation sequencing (NGS) approach to screen for genome-wide nuclear microsatellite loci in the common (Indian) myna, Acridotheres tristis. In addition, markers previously developed for other Sturnidae species were tested for cross-amplification in A. tristis. In total, we identified 20 loci from NGS data and tested 26 loci for cross-amplification. Out of all loci (NGS developed and cross-amplified), 16 unlinked loci showed polymorphism, ranging from 2 to 9 alleles per locus. Test individuals were obtained from the invasive ranges of common myna in South Africa. Overall, expected and observed heterozygosities ranged from 0.089 to 0.802 and from 0.094 to 0.906, respectively. These markers will be used to shed light on invasion genetics and landscape-scale dynamics of invasive A. tristis in South Africa.",

keywords = "Acridotheres tristis, Common myna, Invasive species, Microsatellite",

author = "C{\'e}cile Berthouly-Salazar and Phillip Cassey and {van Vuuren}, {Bettine Jansen} and {van Rensburg}, {Berndt Janse} and Cang Hui and Gardner, {Michael G.} and {Le Roux}, {Johannes J.}",

year = "2012",

month = sep,

doi = "10.1007/s12686-012-9607-8",

language = "English",

volume = "4",

pages = "621--624",

journal = "Conservation Genetics Resources",

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Development and characterization of 13 new, and cross amplification of 3, polymorphic nuclear microsatellite loci in the common myna (Acridotheres tristis). / Berthouly-Salazar, Cécile; Cassey, Phillip; van Vuuren, Bettine Jansen et al.
In: Conservation Genetics Resources, Vol. 4, No. 3, 09.2012, p. 621-624.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development and characterization of 13 new, and cross amplification of 3, polymorphic nuclear microsatellite loci in the common myna (Acridotheres tristis)

AU - Berthouly-Salazar, Cécile

AU - Cassey, Phillip

AU - van Vuuren, Bettine Jansen

AU - van Rensburg, Berndt Janse

AU - Hui, Cang

AU - Gardner, Michael G.

AU - Le Roux, Johannes J.

PY - 2012/9

Y1 - 2012/9

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AB - We used a next generation sequencing (NGS) approach to screen for genome-wide nuclear microsatellite loci in the common (Indian) myna, Acridotheres tristis. In addition, markers previously developed for other Sturnidae species were tested for cross-amplification in A. tristis. In total, we identified 20 loci from NGS data and tested 26 loci for cross-amplification. Out of all loci (NGS developed and cross-amplified), 16 unlinked loci showed polymorphism, ranging from 2 to 9 alleles per locus. Test individuals were obtained from the invasive ranges of common myna in South Africa. Overall, expected and observed heterozygosities ranged from 0.089 to 0.802 and from 0.094 to 0.906, respectively. These markers will be used to shed light on invasion genetics and landscape-scale dynamics of invasive A. tristis in South Africa.

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KW - Common myna

KW - Invasive species

KW - Microsatellite

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Development and characterization of 13 new, and cross amplification of 3, polymorphic nuclear microsatellite loci in the common myna (Acridotheres tristis)

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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