Detection of the 9-kDa vitamin D-dependent calbindin gene in a fruit bat (Rousettus aegyptiacus) fibroblast cell line

Heidi Venter; Keith L. Manchester

doi:10.1016/0305-0491(93)90292-D

Detection of the 9-kDa vitamin D-dependent calbindin gene in a fruit bat (Rousettus aegyptiacus) fibroblast cell line

Heidi Venter, Keith L. Manchester

University of the Witwatersrand

Research output: Contribution to journal › Article › peer-review

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Abstract

1. 1. A fibroblast cell line was established from skin and lung tissue of the fruit bat (Rousettus aegyptiacus). 2. 2. Genomic DNA, isolated from the fibroblast cells, was restriction digested and probed for the 9-kDa calbindin gene with a 180 bae-pair fragment of mouse-calbindin-D9K cDNA. Genomic DNA from rats and humans was similarly probed. 3. 3. Hybridization and thus homology was observed between the mouse probe and DNA from the rat and the fruit bat, but not the human. 4. 4. The results are discussed in relation to the detection of calbindin-D9K in the fruit bat despite very low circulating levels of its inducer 1,25(OH)₂D₃.

Original language	English
Pages (from-to)	629-634
Number of pages	6
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	104
Issue number	3
DOIs	https://doi.org/10.1016/0305-0491(93)90292-D
Publication status	Published - Mar 1993
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Physiology
Molecular Biology

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title = "Detection of the 9-kDa vitamin D-dependent calbindin gene in a fruit bat (Rousettus aegyptiacus) fibroblast cell line",

abstract = "1. 1. A fibroblast cell line was established from skin and lung tissue of the fruit bat (Rousettus aegyptiacus). 2. 2. Genomic DNA, isolated from the fibroblast cells, was restriction digested and probed for the 9-kDa calbindin gene with a 180 bae-pair fragment of mouse-calbindin-D9K cDNA. Genomic DNA from rats and humans was similarly probed. 3. 3. Hybridization and thus homology was observed between the mouse probe and DNA from the rat and the fruit bat, but not the human. 4. 4. The results are discussed in relation to the detection of calbindin-D9K in the fruit bat despite very low circulating levels of its inducer 1,25(OH)2D3.",

author = "Heidi Venter and Manchester, {Keith L.}",

year = "1993",

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doi = "10.1016/0305-0491(93)90292-D",

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T1 - Detection of the 9-kDa vitamin D-dependent calbindin gene in a fruit bat (Rousettus aegyptiacus) fibroblast cell line

AU - Venter, Heidi

AU - Manchester, Keith L.

PY - 1993/3

Y1 - 1993/3

N2 - 1. 1. A fibroblast cell line was established from skin and lung tissue of the fruit bat (Rousettus aegyptiacus). 2. 2. Genomic DNA, isolated from the fibroblast cells, was restriction digested and probed for the 9-kDa calbindin gene with a 180 bae-pair fragment of mouse-calbindin-D9K cDNA. Genomic DNA from rats and humans was similarly probed. 3. 3. Hybridization and thus homology was observed between the mouse probe and DNA from the rat and the fruit bat, but not the human. 4. 4. The results are discussed in relation to the detection of calbindin-D9K in the fruit bat despite very low circulating levels of its inducer 1,25(OH)2D3.

AB - 1. 1. A fibroblast cell line was established from skin and lung tissue of the fruit bat (Rousettus aegyptiacus). 2. 2. Genomic DNA, isolated from the fibroblast cells, was restriction digested and probed for the 9-kDa calbindin gene with a 180 bae-pair fragment of mouse-calbindin-D9K cDNA. Genomic DNA from rats and humans was similarly probed. 3. 3. Hybridization and thus homology was observed between the mouse probe and DNA from the rat and the fruit bat, but not the human. 4. 4. The results are discussed in relation to the detection of calbindin-D9K in the fruit bat despite very low circulating levels of its inducer 1,25(OH)2D3.

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Detection of the 9-kDa vitamin D-dependent calbindin gene in a fruit bat (Rousettus aegyptiacus) fibroblast cell line

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