Delivery of ribosome-inactivating protein toxin into cancer cells with shock waves

Tetsuya Kodama; Apostolos G. Doukas; Michael R. Hamblin

doi:10.1016/S0304-3835(02)00503-7

Delivery of ribosome-inactivating protein toxin into cancer cells with shock waves

Tetsuya Kodama, Apostolos G. Doukas, Michael R. Hamblin

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

We report on the use of shock waves delivered by a shock-tube to permeabilize cancer cells and potentiate the cytotoxicity of the type-1 ribosome-inactivating protein, saporin. We studied human colorectal cancer HT29 and ovarian cancer OVCAR-5 cells, and used two different cytotoxicity assays, colony formation and loss of mitochondrial activity. A single shock wave and saporin (10^-9 M) produced significant toxicity not seen with either shock wave or drug alone. Increasing the number of shock waves up to five further increased cytotoxicity. Higher toxicity was seen with the clonogenic assay compared to MTT assay. Shock waves may have applications in promoting cytoplasmic delivery of toxins into cancer cells after intratumoral injection.

Original language	English
Pages (from-to)	69-75
Number of pages	7
Journal	Cancer Letters
Volume	189
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3835(02)00503-7
Publication status	Published - 10 Jan 2003
Externally published	Yes

Keywords

Cancer
Cytoplasmic delivery
Ribosome-inactivating protein
Saporin
Shock tube

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3835(02)00503-7

Cite this

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title = "Delivery of ribosome-inactivating protein toxin into cancer cells with shock waves",

abstract = "We report on the use of shock waves delivered by a shock-tube to permeabilize cancer cells and potentiate the cytotoxicity of the type-1 ribosome-inactivating protein, saporin. We studied human colorectal cancer HT29 and ovarian cancer OVCAR-5 cells, and used two different cytotoxicity assays, colony formation and loss of mitochondrial activity. A single shock wave and saporin (10-9 M) produced significant toxicity not seen with either shock wave or drug alone. Increasing the number of shock waves up to five further increased cytotoxicity. Higher toxicity was seen with the clonogenic assay compared to MTT assay. Shock waves may have applications in promoting cytoplasmic delivery of toxins into cancer cells after intratumoral injection.",

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T1 - Delivery of ribosome-inactivating protein toxin into cancer cells with shock waves

AU - Kodama, Tetsuya

AU - Doukas, Apostolos G.

AU - Hamblin, Michael R.

PY - 2003/1/10

Y1 - 2003/1/10

N2 - We report on the use of shock waves delivered by a shock-tube to permeabilize cancer cells and potentiate the cytotoxicity of the type-1 ribosome-inactivating protein, saporin. We studied human colorectal cancer HT29 and ovarian cancer OVCAR-5 cells, and used two different cytotoxicity assays, colony formation and loss of mitochondrial activity. A single shock wave and saporin (10-9 M) produced significant toxicity not seen with either shock wave or drug alone. Increasing the number of shock waves up to five further increased cytotoxicity. Higher toxicity was seen with the clonogenic assay compared to MTT assay. Shock waves may have applications in promoting cytoplasmic delivery of toxins into cancer cells after intratumoral injection.

AB - We report on the use of shock waves delivered by a shock-tube to permeabilize cancer cells and potentiate the cytotoxicity of the type-1 ribosome-inactivating protein, saporin. We studied human colorectal cancer HT29 and ovarian cancer OVCAR-5 cells, and used two different cytotoxicity assays, colony formation and loss of mitochondrial activity. A single shock wave and saporin (10-9 M) produced significant toxicity not seen with either shock wave or drug alone. Increasing the number of shock waves up to five further increased cytotoxicity. Higher toxicity was seen with the clonogenic assay compared to MTT assay. Shock waves may have applications in promoting cytoplasmic delivery of toxins into cancer cells after intratumoral injection.

KW - Cancer

KW - Cytoplasmic delivery

KW - Ribosome-inactivating protein

KW - Saporin

KW - Shock tube

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DO - 10.1016/S0304-3835(02)00503-7

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AN - SCOPUS:0037427558

SN - 0304-3835

VL - 189

SP - 69

EP - 75

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Delivery of ribosome-inactivating protein toxin into cancer cells with shock waves

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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