Cyanide Biodegradation by Trichoderma harzianum and Cyanide Hydratase Network Analysis

Narges Malmir, Mohammadreza Zamani, Mostafa Motallebi, Najaf Allahyari Fard, Lukhanyo Mekuto

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Cyanide is a poisonous and dangerous chemical that binds to metals in metalloenzymes, especially cytochrome C oxidase and, thus, interferes with their functionalities. Different pathways and enzymes are involved during cyanide biodegradation, and cyanide hydratase is one of the enzymes that is involved in such a process. In this study, cyanide resistance and cyanide degradation were studied using 24 fungal strains in order to find the strain with the best capacity for cyanide bioremediation. To confirm the capacity of the tested strains, cyano-bioremediation and the presence of the gene that is responsible for the cyanide detoxification was assessed. From the tested organisms, Trichoderma harzianum (T. harzianum) had a significant capability to resist and degrade cyanide at a 15 mM concentration, where it achieved an efficiency of 75% in 7 days. The gene network analysis of enzymes that are involved in cyanide degradation revealed the involvement of cyanide hydratase, dipeptidase, carbon–nitrogen hydrolase-like protein, and ATP adenylyltransferase. This study revealed that T. harzianum was more efficient in degrading cyanide than the other tested fungal organisms, and molecular analysis confirmed the experimental observations.

Original languageEnglish
Article number3336
JournalMolecules
Volume27
Issue number10
DOIs
Publication statusPublished - 1 May 2022

Keywords

  • biodegradation
  • cyanide
  • cyanide hydratase (CHT)
  • Trichoderma harzianum

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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