TY - JOUR
T1 - Combined therapy of adipose-derived stem cells and photobiomodulation on accelerated bone healing of a critical size defect in an osteoporotic rat model
AU - Asgari, Mehrdad
AU - Gazor, Rouhallah
AU - Abdollahifar, Mohammad Amin
AU - Fadaei Fathabady, Fatemeh
AU - Zare, Fatemeh
AU - Norouzian, Mohsen
AU - Amini, Abdollah
AU - Khosravipour, Armin
AU - Kiani, Pejman
AU - Atashgah, Rahimeh B.
AU - Rezaei, Fatemehsadat
AU - Ghoreishi, Seyed Kamran
AU - Chien, Sufan
AU - Hamblin, Michael R.
AU - Bayat, Mohammad
N1 - Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2020/9/10
Y1 - 2020/9/10
N2 - We investigated the impact of human demineralized bone matrix (hDBM) plus adipose-derived stem cells (hADS) plus photobiomodulation (PBM) on a critical-sized femoral defect (CSFD) in ovariectomy induced osteoporosis in rats. There were 6 groups as follows. In group 1 (control, C), only CSFDs were created. Groups 2–6 were implanted with DBM into the CSFD (DBM-CSFD). In group 2 (S), only DBM was transplanted into the CSFD. In group 3 (S + PBM), the DBM-CSFDs were treated with PBM. In group 4, the DBM-CSFDs were treated with alendronate (S + ALN). In group 5, ADSs were seeded into DBM-CSFD (S + ADS). In group 6, ADSs were seeded into DBM-CSFD and the CSFDs were treated with PBM (S + PBM + ADS). At week eight (catabolic phase of bone repair), the S + ALN, S + PBM + ADS, S + PBM, and S + ADS groups all had significantly increased bone strength than the S group (ANOVA, p = 0.000). The S + PBM, S + PBM + ADS, and S + ADS groups had significantly increased Hounsfield unit than the S group (ANOVA, p = 0.000). ALN, ADS, and PBM significantly increased healed bone strength in an experimental model of DBM-treated CSFD in the catabolic phase of bone healing in osteoporotic rats. However, ALN alone and PBM plus ADS were superior to the other protocols.
AB - We investigated the impact of human demineralized bone matrix (hDBM) plus adipose-derived stem cells (hADS) plus photobiomodulation (PBM) on a critical-sized femoral defect (CSFD) in ovariectomy induced osteoporosis in rats. There were 6 groups as follows. In group 1 (control, C), only CSFDs were created. Groups 2–6 were implanted with DBM into the CSFD (DBM-CSFD). In group 2 (S), only DBM was transplanted into the CSFD. In group 3 (S + PBM), the DBM-CSFDs were treated with PBM. In group 4, the DBM-CSFDs were treated with alendronate (S + ALN). In group 5, ADSs were seeded into DBM-CSFD (S + ADS). In group 6, ADSs were seeded into DBM-CSFD and the CSFDs were treated with PBM (S + PBM + ADS). At week eight (catabolic phase of bone repair), the S + ALN, S + PBM + ADS, S + PBM, and S + ADS groups all had significantly increased bone strength than the S group (ANOVA, p = 0.000). The S + PBM, S + PBM + ADS, and S + ADS groups had significantly increased Hounsfield unit than the S group (ANOVA, p = 0.000). ALN, ADS, and PBM significantly increased healed bone strength in an experimental model of DBM-treated CSFD in the catabolic phase of bone healing in osteoporotic rats. However, ALN alone and PBM plus ADS were superior to the other protocols.
KW - Demineralized bone matrix
KW - Fracture healing
KW - Human adipose-derived stem cells
KW - Osteoporosis
KW - Photobiomodulation
UR - https://www.scopus.com/pages/publications/85089279022
U2 - 10.1016/j.bbrc.2020.06.023
DO - 10.1016/j.bbrc.2020.06.023
M3 - Article
C2 - 32828282
AN - SCOPUS:85089279022
SN - 0006-291X
VL - 530
SP - 173
EP - 180
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -