Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells

Maryam Ghaffari; Gholamreza Dehghan; Behzad Baradaran; Amir Zarebkohan; Behzad Mansoori; Jafar Soleymani; Jafar Ezzati Nazhad Dolatabadi; Michael R. Hamblin

doi:10.1016/j.colsurfb.2019.110762

Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells

Maryam Ghaffari
, Gholamreza Dehghan
, Behzad Baradaran
, Amir Zarebkohan
, Behzad Mansoori
, Jafar Soleymani
, Jafar Ezzati Nazhad Dolatabadi
, Michael R. Hamblin

Laser Research Centre

Tabriz University of Medical Sciences
University of Tabriz
Massachusetts General Hospital
Harvard University

Research output: Contribution to journal › Article › peer-review

141 Citations (Scopus)

Abstract

Co-delivery of therapeutic agents and small interfering RNA (siRNA) can be achieved by a suitable nanovehicle. In this work, the solubility and bioavailability of curcumin (Cur) were enhanced by entrapment in a polyamidoamine (PAMAM) dendrimer, and a polyplex was formed by grafting Bcl-2 siRNA onto the surface amine groups to produce PAMAM-Cur/Bcl-2 siRNA nanoparticles (NPs). The synthesized polyplex NPs had a particle size of ∼180 nm, and high Cur loading content of ∼82 wt%. Moreover, the PAMAM-Cur/Bcl-2 siRNA NPs showed more effective cellular uptake, and higher inhibition of tumor cell proliferation compared to PAMAM-Cur nanoformulation and free Cur, due to the combined effect of co-delivery of Cur and Bcl-2 siRNA. The newly described PAMAM-Cur/Bcl-2 siRNA polyplex NPs could be a promising co-delivery nanovehicle.

Original language	English
Article number	110762
Journal	Colloids and Surfaces B: Biointerfaces
Volume	188
DOIs	https://doi.org/10.1016/j.colsurfb.2019.110762
Publication status	Published - Apr 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Bcl-2 siRNA
Co-delivery
Curcumin
HeLa cancer cells
Nanoparticles
PAMAM dendrimer

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2019.110762

Cite this

Ghaffari, M., Dehghan, G., Baradaran, B., Zarebkohan, A., Mansoori, B., Soleymani, J., Ezzati Nazhad Dolatabadi, J., & Hamblin, M. R. (2020). Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells. Colloids and Surfaces B: Biointerfaces, 188, Article 110762. https://doi.org/10.1016/j.colsurfb.2019.110762

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title = "Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells",

abstract = "Co-delivery of therapeutic agents and small interfering RNA (siRNA) can be achieved by a suitable nanovehicle. In this work, the solubility and bioavailability of curcumin (Cur) were enhanced by entrapment in a polyamidoamine (PAMAM) dendrimer, and a polyplex was formed by grafting Bcl-2 siRNA onto the surface amine groups to produce PAMAM-Cur/Bcl-2 siRNA nanoparticles (NPs). The synthesized polyplex NPs had a particle size of ∼180 nm, and high Cur loading content of ∼82 wt\%. Moreover, the PAMAM-Cur/Bcl-2 siRNA NPs showed more effective cellular uptake, and higher inhibition of tumor cell proliferation compared to PAMAM-Cur nanoformulation and free Cur, due to the combined effect of co-delivery of Cur and Bcl-2 siRNA. The newly described PAMAM-Cur/Bcl-2 siRNA polyplex NPs could be a promising co-delivery nanovehicle.",

keywords = "Bcl-2 siRNA, Co-delivery, Curcumin, HeLa cancer cells, Nanoparticles, PAMAM dendrimer",

author = "Maryam Ghaffari and Gholamreza Dehghan and Behzad Baradaran and Amir Zarebkohan and Behzad Mansoori and Jafar Soleymani and \{Ezzati Nazhad Dolatabadi\}, Jafar and Hamblin, \{Michael R.\}",

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year = "2020",

month = apr,

doi = "10.1016/j.colsurfb.2019.110762",

language = "English",

volume = "188",

journal = "Colloids and Surfaces B: Biointerfaces",

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AU - Ghaffari, Maryam

AU - Dehghan, Gholamreza

AU - Baradaran, Behzad

AU - Zarebkohan, Amir

AU - Mansoori, Behzad

AU - Soleymani, Jafar

AU - Ezzati Nazhad Dolatabadi, Jafar

AU - Hamblin, Michael R.

PY - 2020/4

Y1 - 2020/4

N2 - Co-delivery of therapeutic agents and small interfering RNA (siRNA) can be achieved by a suitable nanovehicle. In this work, the solubility and bioavailability of curcumin (Cur) were enhanced by entrapment in a polyamidoamine (PAMAM) dendrimer, and a polyplex was formed by grafting Bcl-2 siRNA onto the surface amine groups to produce PAMAM-Cur/Bcl-2 siRNA nanoparticles (NPs). The synthesized polyplex NPs had a particle size of ∼180 nm, and high Cur loading content of ∼82 wt%. Moreover, the PAMAM-Cur/Bcl-2 siRNA NPs showed more effective cellular uptake, and higher inhibition of tumor cell proliferation compared to PAMAM-Cur nanoformulation and free Cur, due to the combined effect of co-delivery of Cur and Bcl-2 siRNA. The newly described PAMAM-Cur/Bcl-2 siRNA polyplex NPs could be a promising co-delivery nanovehicle.

AB - Co-delivery of therapeutic agents and small interfering RNA (siRNA) can be achieved by a suitable nanovehicle. In this work, the solubility and bioavailability of curcumin (Cur) were enhanced by entrapment in a polyamidoamine (PAMAM) dendrimer, and a polyplex was formed by grafting Bcl-2 siRNA onto the surface amine groups to produce PAMAM-Cur/Bcl-2 siRNA nanoparticles (NPs). The synthesized polyplex NPs had a particle size of ∼180 nm, and high Cur loading content of ∼82 wt%. Moreover, the PAMAM-Cur/Bcl-2 siRNA NPs showed more effective cellular uptake, and higher inhibition of tumor cell proliferation compared to PAMAM-Cur nanoformulation and free Cur, due to the combined effect of co-delivery of Cur and Bcl-2 siRNA. The newly described PAMAM-Cur/Bcl-2 siRNA polyplex NPs could be a promising co-delivery nanovehicle.

KW - Bcl-2 siRNA

KW - Co-delivery

KW - Curcumin

KW - HeLa cancer cells

KW - Nanoparticles

KW - PAMAM dendrimer

UR - https://www.scopus.com/pages/publications/85078730655

U2 - 10.1016/j.colsurfb.2019.110762

DO - 10.1016/j.colsurfb.2019.110762

M3 - Article

C2 - 31911391

AN - SCOPUS:85078730655

SN - 0927-7765

VL - 188

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

M1 - 110762

ER -

Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

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