Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells

Maryam Ghaffari, Gholamreza Dehghan, Behzad Baradaran, Amir Zarebkohan, Behzad Mansoori, Jafar Soleymani, Jafar Ezzati Nazhad Dolatabadi, Michael R. Hamblin

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

Co-delivery of therapeutic agents and small interfering RNA (siRNA) can be achieved by a suitable nanovehicle. In this work, the solubility and bioavailability of curcumin (Cur) were enhanced by entrapment in a polyamidoamine (PAMAM) dendrimer, and a polyplex was formed by grafting Bcl-2 siRNA onto the surface amine groups to produce PAMAM-Cur/Bcl-2 siRNA nanoparticles (NPs). The synthesized polyplex NPs had a particle size of ∼180 nm, and high Cur loading content of ∼82 wt%. Moreover, the PAMAM-Cur/Bcl-2 siRNA NPs showed more effective cellular uptake, and higher inhibition of tumor cell proliferation compared to PAMAM-Cur nanoformulation and free Cur, due to the combined effect of co-delivery of Cur and Bcl-2 siRNA. The newly described PAMAM-Cur/Bcl-2 siRNA polyplex NPs could be a promising co-delivery nanovehicle.

Original languageEnglish
Article number110762
JournalColloids and Surfaces B: Biointerfaces
Volume188
DOIs
Publication statusPublished - Apr 2020

Keywords

  • Bcl-2 siRNA
  • Co-delivery
  • Curcumin
  • HeLa cancer cells
  • Nanoparticles
  • PAMAM dendrimer

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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