Characterization of 14 polymorphic microsatellite loci developed for an Afrotherian species endemic to southern Africa, Elephantulus myurus (Macroscelidea: Macroscelididae)

Sasha Hoffmann, Heather J. Webster, Peter R. Teske, Heike Lutermann, Nigel C. Bennett, Bettine Jansen van Vuuren

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Fourteen microsatellite loci were developed for the eastern rock sengi, Elephantulus myurus Thomas & Schwann, 1906 by incorporating genetic diversity from across its range in South Africa. Sengis are small mammals belonging to the order Macroscelidea, which comprises 19 species, all of which are endemic to Africa. The loci were amplified in 66 individuals from six localities. An average of 10.5 alleles per locus were identified, with observed and expected heterozygosity values ranging from 0.081 to 0.909 and 0.404 to 0.911, respectively. We also investigated cross-species amplification within the family and found variation in amplification success for five different species. The preliminary results from these amplification efforts could aid further studies into aspects of species diversity and biology. The markers described here represent the first set of variable nuclear markers for the genus Elephantulus, and together with a set of 8 recently developed markers for Rhynchocyon petersi, Bocage 1880, the first markers for the order Macroscelidea.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalApplied Entomology and Zoology
Volume52
Issue number1
DOIs
Publication statusPublished - 1 Feb 2017

Keywords

  • 454-Sequencing
  • Afrotheria
  • Elephantulus
  • Macroscelidea
  • Multiplex
  • SSR

ASJC Scopus subject areas

  • Insect Science

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