Cationic photoimmunoconjugates between monoclonal antibodies and hematoporphyrin: Selective photodestruction of ovarian cancer cells

Michael R. Hamblin; Michael P. Bamberg; Jaimie L. Miller; Tayyaba Hasan

doi:10.1364/AO.37.007184

Cationic photoimmunoconjugates between monoclonal antibodies and hematoporphyrin: Selective photodestruction of ovarian cancer cells

Michael R. Hamblin
, Michael P. Bamberg
, Jaimie L. Miller
, Tayyaba Hasan

Massachusetts General Hospital

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The photosensitizer hematoporphyrin (HP) was site specifically attached to a murine monoclonal antibody (MAb) fragment OC125F(ab^’)₂ directed against ovarian cancer cells and to nonspecific rabbit immunoglobulin G. The photoimmunoconjugates were positively charged and were purified by column chromatography. The OC125F(ab^’)₂ conjugate retained immunoreactivity with human ovarian cancer cells, and the binding was competed with unmodified MAb. Phototoxicity paralleled the cellular uptake with the OC125F(ab^’)₂ conjugate and the light showing selective killing of target cells compared with nontarget cells. Nontargeted conjugates and free HP produced lower levels of phototoxicity and showed no selectivity.

Original language	English
Pages (from-to)	7184-7192
Number of pages	9
Journal	Applied Optics
Volume	37
Issue number	31
DOIs	https://doi.org/10.1364/AO.37.007184
Publication status	Published - 1 Nov 1998
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

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10.1364/AO.37.007184

Cite this

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title = "Cationic photoimmunoconjugates between monoclonal antibodies and hematoporphyrin: Selective photodestruction of ovarian cancer cells",

abstract = "The photosensitizer hematoporphyrin (HP) was site specifically attached to a murine monoclonal antibody (MAb) fragment OC125F(ab{\textquoteright})2 directed against ovarian cancer cells and to nonspecific rabbit immunoglobulin G. The photoimmunoconjugates were positively charged and were purified by column chromatography. The OC125F(ab{\textquoteright})2 conjugate retained immunoreactivity with human ovarian cancer cells, and the binding was competed with unmodified MAb. Phototoxicity paralleled the cellular uptake with the OC125F(ab{\textquoteright})2 conjugate and the light showing selective killing of target cells compared with nontarget cells. Nontargeted conjugates and free HP produced lower levels of phototoxicity and showed no selectivity.",

author = "Hamblin, \{Michael R.\} and Bamberg, \{Michael P.\} and Miller, \{Jaimie L.\} and Tayyaba Hasan",

year = "1998",

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doi = "10.1364/AO.37.007184",

language = "English",

volume = "37",

pages = "7184--7192",

journal = "Applied Optics",

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publisher = "Optica Publishing Group",

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T1 - Cationic photoimmunoconjugates between monoclonal antibodies and hematoporphyrin

T2 - Selective photodestruction of ovarian cancer cells

AU - Hamblin, Michael R.

AU - Bamberg, Michael P.

AU - Miller, Jaimie L.

AU - Hasan, Tayyaba

PY - 1998/11/1

Y1 - 1998/11/1

N2 - The photosensitizer hematoporphyrin (HP) was site specifically attached to a murine monoclonal antibody (MAb) fragment OC125F(ab’)2 directed against ovarian cancer cells and to nonspecific rabbit immunoglobulin G. The photoimmunoconjugates were positively charged and were purified by column chromatography. The OC125F(ab’)2 conjugate retained immunoreactivity with human ovarian cancer cells, and the binding was competed with unmodified MAb. Phototoxicity paralleled the cellular uptake with the OC125F(ab’)2 conjugate and the light showing selective killing of target cells compared with nontarget cells. Nontargeted conjugates and free HP produced lower levels of phototoxicity and showed no selectivity.

AB - The photosensitizer hematoporphyrin (HP) was site specifically attached to a murine monoclonal antibody (MAb) fragment OC125F(ab’)2 directed against ovarian cancer cells and to nonspecific rabbit immunoglobulin G. The photoimmunoconjugates were positively charged and were purified by column chromatography. The OC125F(ab’)2 conjugate retained immunoreactivity with human ovarian cancer cells, and the binding was competed with unmodified MAb. Phototoxicity paralleled the cellular uptake with the OC125F(ab’)2 conjugate and the light showing selective killing of target cells compared with nontarget cells. Nontargeted conjugates and free HP produced lower levels of phototoxicity and showed no selectivity.

UR - https://www.scopus.com/pages/publications/0001635330

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DO - 10.1364/AO.37.007184

M3 - Article

AN - SCOPUS:0001635330

SN - 1559-128X

VL - 37

SP - 7184

EP - 7192

JO - Applied Optics

JF - Applied Optics

IS - 31

ER -

Cationic photoimmunoconjugates between monoclonal antibodies and hematoporphyrin: Selective photodestruction of ovarian cancer cells

Abstract

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