Cationic photoimmunoconjugates between monoclonal antibodies and hematoporphyrin: Selective photodestruction of ovarian cancer cells

Michael R. Hamblin, Michael P. Bamberg, Jaimie L. Miller, Tayyaba Hasan

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The photosensitizer hematoporphyrin (HP) was site specifically attached to a murine monoclonal antibody (MAb) fragment OC125F(ab)2 directed against ovarian cancer cells and to nonspecific rabbit immunoglobulin G. The photoimmunoconjugates were positively charged and were purified by column chromatography. The OC125F(ab)2 conjugate retained immunoreactivity with human ovarian cancer cells, and the binding was competed with unmodified MAb. Phototoxicity paralleled the cellular uptake with the OC125F(ab)2 conjugate and the light showing selective killing of target cells compared with nontarget cells. Nontargeted conjugates and free HP produced lower levels of phototoxicity and showed no selectivity.

Original languageEnglish
Pages (from-to)7184-7192
Number of pages9
JournalApplied Optics
Volume37
Issue number31
DOIs
Publication statusPublished - 1 Nov 1998
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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