Calmodulin from Citrus sinensis: Purification and characterization

Ian A. Dubery; Johannes C. Schabort

doi:10.1016/S0031-9422(00)81476-4

Calmodulin from Citrus sinensis: Purification and characterization

Ian A. Dubery
, Johannes C. Schabort

South African Nuclear Energy Corporation

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A protein identifiable as calmodulin has been isolated from the fruit tissue of Citrus sinensis (cv. Valencia). This protein is relatively heat-stable, binds to hydrophobic gels in a calcium-dependent fashion and to antibody against rat testis calmodulin, and stimulates calmodulin-deficient cAMP phosphodiesterase. The protein has an isoelectric point of 3.7 and M_rs of 14500 and 17000 in the presence and absence of Ca²⁺, respectively. Based on its migration on SDS-PAGE gels, its UV absorption spectrum and its amino acid composition, calmodulin from citrus fruit is essentially identical to calmodulin isolated from other plant tissues, e.g. leaves and seeds.

Original language	English
Pages (from-to)	37-40
Number of pages	4
Journal	Phytochemistry
Volume	26
Issue number	1
DOIs	https://doi.org/10.1016/S0031-9422(00)81476-4
Publication status	Published - 23 Dec 1986
Externally published	Yes

Keywords

Citrus sinensis, Rutaceae
calmodulin.

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

Access to Document

10.1016/S0031-9422(00)81476-4

Cite this

@article{a654e8bbac1140a18ec9ad29095def56,

title = "Calmodulin from Citrus sinensis: Purification and characterization",

abstract = "A protein identifiable as calmodulin has been isolated from the fruit tissue of Citrus sinensis (cv. Valencia). This protein is relatively heat-stable, binds to hydrophobic gels in a calcium-dependent fashion and to antibody against rat testis calmodulin, and stimulates calmodulin-deficient cAMP phosphodiesterase. The protein has an isoelectric point of 3.7 and Mrs of 14500 and 17000 in the presence and absence of Ca2+, respectively. Based on its migration on SDS-PAGE gels, its UV absorption spectrum and its amino acid composition, calmodulin from citrus fruit is essentially identical to calmodulin isolated from other plant tissues, e.g. leaves and seeds.",

keywords = "Citrus sinensis, Rutaceae, calmodulin.",

author = "Dubery, \{Ian A.\} and Schabort, \{Johannes C.\}",

year = "1986",

month = dec,

day = "23",

doi = "10.1016/S0031-9422(00)81476-4",

language = "English",

volume = "26",

pages = "37--40",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Elsevier Ltd.",

number = "1",

}

TY - JOUR

T1 - Calmodulin from Citrus sinensis

T2 - Purification and characterization

AU - Dubery, Ian A.

AU - Schabort, Johannes C.

PY - 1986/12/23

Y1 - 1986/12/23

N2 - A protein identifiable as calmodulin has been isolated from the fruit tissue of Citrus sinensis (cv. Valencia). This protein is relatively heat-stable, binds to hydrophobic gels in a calcium-dependent fashion and to antibody against rat testis calmodulin, and stimulates calmodulin-deficient cAMP phosphodiesterase. The protein has an isoelectric point of 3.7 and Mrs of 14500 and 17000 in the presence and absence of Ca2+, respectively. Based on its migration on SDS-PAGE gels, its UV absorption spectrum and its amino acid composition, calmodulin from citrus fruit is essentially identical to calmodulin isolated from other plant tissues, e.g. leaves and seeds.

AB - A protein identifiable as calmodulin has been isolated from the fruit tissue of Citrus sinensis (cv. Valencia). This protein is relatively heat-stable, binds to hydrophobic gels in a calcium-dependent fashion and to antibody against rat testis calmodulin, and stimulates calmodulin-deficient cAMP phosphodiesterase. The protein has an isoelectric point of 3.7 and Mrs of 14500 and 17000 in the presence and absence of Ca2+, respectively. Based on its migration on SDS-PAGE gels, its UV absorption spectrum and its amino acid composition, calmodulin from citrus fruit is essentially identical to calmodulin isolated from other plant tissues, e.g. leaves and seeds.

KW - Citrus sinensis, Rutaceae

KW - calmodulin.

UR - https://www.scopus.com/pages/publications/38249040702

U2 - 10.1016/S0031-9422(00)81476-4

DO - 10.1016/S0031-9422(00)81476-4

M3 - Article

AN - SCOPUS:38249040702

SN - 0031-9422

VL - 26

SP - 37

EP - 40

JO - Phytochemistry

JF - Phytochemistry

IS - 1

ER -

Calmodulin from Citrus sinensis: Purification and characterization

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this