Calmodulin from Citrus sinensis: Purification and characterization

Ian A. Dubery, Johannes C. Schabort

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


A protein identifiable as calmodulin has been isolated from the fruit tissue of Citrus sinensis (cv. Valencia). This protein is relatively heat-stable, binds to hydrophobic gels in a calcium-dependent fashion and to antibody against rat testis calmodulin, and stimulates calmodulin-deficient cAMP phosphodiesterase. The protein has an isoelectric point of 3.7 and Mrs of 14500 and 17000 in the presence and absence of Ca2+, respectively. Based on its migration on SDS-PAGE gels, its UV absorption spectrum and its amino acid composition, calmodulin from citrus fruit is essentially identical to calmodulin isolated from other plant tissues, e.g. leaves and seeds.

Original languageEnglish
Pages (from-to)37-40
Number of pages4
Issue number1
Publication statusPublished - 23 Dec 1986
Externally publishedYes


  • Citrus sinensis, Rutaceae
  • calmodulin.

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture


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