Burkholderia dilworthii sp. nov., isolated from Lebeckia ambigua root nodules

Sofie E. De Meyer; Margo Cnockaert; Julie K. Ardley; Ben Erik Van Wyk; Peter A. Vandamme; John G. Howieson

doi:10.1099/ijs.0.058602-0

Burkholderia dilworthii sp. nov., isolated from Lebeckia ambigua root nodules

Sofie E. De Meyer, Margo Cnockaert, Julie K. Ardley, Ben Erik Van Wyk, Peter A. Vandamme, John G. Howieson

Botany and Plant Biotechnology

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM3556^T being most closely related to Burkholderia caledonica LMG 23644^T (98.70 % 16S rRNA gene sequence similarity) and Burkholderia rhynchosiae WSM3937^T (98.50 %). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes gyrB and recA. Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus Burkholderia. Results of DNA-DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name Burkholderia dilworthii sp. nov. is proposed, with the type strain WSM3556^T (= LMG 27173^T = HAMBI 3353^T).

Original language	English
Article number	058602
Pages (from-to)	1090-1095
Number of pages	6
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	64
Issue number	PART 4
DOIs	https://doi.org/10.1099/ijs.0.058602-0
Publication status	Published - 2014

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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title = "Burkholderia dilworthii sp. nov., isolated from Lebeckia ambigua root nodules",

abstract = "Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM3556T being most closely related to Burkholderia caledonica LMG 23644T (98.70 % 16S rRNA gene sequence similarity) and Burkholderia rhynchosiae WSM3937T (98.50 %). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes gyrB and recA. Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus Burkholderia. Results of DNA-DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name Burkholderia dilworthii sp. nov. is proposed, with the type strain WSM3556T (= LMG 27173T = HAMBI 3353T).",

author = "{De Meyer}, {Sofie E.} and Margo Cnockaert and Ardley, {Julie K.} and {Van Wyk}, {Ben Erik} and Vandamme, {Peter A.} and Howieson, {John G.}",

year = "2014",

doi = "10.1099/ijs.0.058602-0",

language = "English",

volume = "64",

pages = "1090--1095",

journal = "International Journal of Systematic and Evolutionary Microbiology",

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T1 - Burkholderia dilworthii sp. nov., isolated from Lebeckia ambigua root nodules

AU - De Meyer, Sofie E.

AU - Cnockaert, Margo

AU - Ardley, Julie K.

AU - Van Wyk, Ben Erik

AU - Vandamme, Peter A.

AU - Howieson, John G.

PY - 2014

Y1 - 2014

N2 - Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM3556T being most closely related to Burkholderia caledonica LMG 23644T (98.70 % 16S rRNA gene sequence similarity) and Burkholderia rhynchosiae WSM3937T (98.50 %). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes gyrB and recA. Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus Burkholderia. Results of DNA-DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name Burkholderia dilworthii sp. nov. is proposed, with the type strain WSM3556T (= LMG 27173T = HAMBI 3353T).

AB - Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM3556T being most closely related to Burkholderia caledonica LMG 23644T (98.70 % 16S rRNA gene sequence similarity) and Burkholderia rhynchosiae WSM3937T (98.50 %). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes gyrB and recA. Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus Burkholderia. Results of DNA-DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name Burkholderia dilworthii sp. nov. is proposed, with the type strain WSM3556T (= LMG 27173T = HAMBI 3353T).

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SN - 1466-5026

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JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - PART 4

M1 - 058602

ER -

Burkholderia dilworthii sp. nov., isolated from Lebeckia ambigua root nodules

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