Biomarkers of skeletal muscle injury in the rat

Nivaldo A. Parizotto, Natalia C. Rodrigues, Paulo Sergio Bossini, Michael R. Hamblin

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

We can mark different aspects of metabolism in the cell or tissue using immunohistochemistry and immunofluorescence methods to identify protein and amino acid sequences in the tissues, including muscles. It can used to show how is the phase of the many processes are the tissues. The process is not difficult to do and we can identify the presence of many antibodies from a variety of conditions, like inflammation and repair process in the muscle. It is very interesting to see the presence of stem cells of the muscle tissue, like satellite cells, using this kind of technique. For the reader, have a quick passage on the steps to be followed for processing and observation of the muscle tissue so that they can follow not only the difficulties but also the facilities of the IHC method with biomarkers. In this chapter, the reader could find some results using the method to investigate the muscle lesion and repair.

Original languageEnglish
Title of host publicationTechnical Aspects of Toxicological Immunohistochemistry
Subtitle of host publicationSystem Specific Biomarkers
PublisherSpringer New York
Pages215-226
Number of pages12
ISBN (Electronic)9781493915163
ISBN (Print)9781493915156
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Keywords

  • Immunofluorescence
  • Immunohistochemistry
  • Muscle lesion

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • General Medicine
  • Pharmacology, Toxicology and Pharmaceutics (all)
  • General Agricultural and Biological Sciences

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