Biochemical genetic variation in four wild populations of Aspalathus linearis (Rooibos Tea)

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27 Citations (Scopus)

Abstract

Four morphologically different populations of Aspalathus linearis were examined by horizontal starch gel electrophoresis to assess levels and patterns of genetic variation and to estimate the amount of genetic differentiation within and between populations. Leaf extracts were surveyed for 13 enzymes, and gene products revealed genetic variation at six (40%) of 15 protein coding loci. The percentages of polymorphic loci range from 6.67 to 26.67% (0.95 criterion), values of 1.07-1.40 were obtained for the mean number of alleles per locus, and average heterozygosities per locus were calculated at 0.016-0.096. The mean genotypic distance index (Nei, 1978) between populations was 0.034. Enzyme electrophoresis appears to be a suitable method for studying genetic variation in A. linearis.

Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalBiochemical Systematics and Ecology
Volume23
Issue number3
DOIs
Publication statusPublished - Apr 1995

Keywords

  • Aspalathus linearis
  • Fabaceae
  • Rooibos tea
  • electrophoresis
  • genetic distance
  • genetic diversity

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

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