Antimicrobial photodynamic therapy with RLP068 kills methicillin-resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion

Daniela Vecchio; Tianhong Dai; Liyi Huang; Lia Fantetti; Gabrio Roncucci; Michael R. Hamblin

doi:10.1002/jbio.201200121

Antimicrobial photodynamic therapy with RLP068 kills methicillin-resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion

Daniela Vecchio, Tianhong Dai, Liyi Huang, Lia Fantetti, Gabrio Roncucci, Michael R. Hamblin

Research output: Contribution to journal › Article › peer-review

79 Citations (Scopus)

Abstract

Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse skin abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process.

Original language	English
Pages (from-to)	733-742
Number of pages	10
Journal	Journal of Biophotonics
Volume	6
Issue number	9
DOIs	https://doi.org/10.1002/jbio.201200121
Publication status	Published - Sept 2013
Externally published	Yes

Keywords

Antimicrobial photodynamic therapy
Cationic zinc phthalocyanine bioluminescence imaging
Methicillin resistant Staphylococcus aureus
Skin abrasion
Skin and soft tissue infection
Wound healing

ASJC Scopus subject areas

General Chemistry
General Materials Science
General Biochemistry,Genetics and Molecular Biology
General Engineering
General Physics and Astronomy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jbio.201200121

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Vecchio, D., Dai, T., Huang, L., Fantetti, L., Roncucci, G., & Hamblin, M. R. (2013). Antimicrobial photodynamic therapy with RLP068 kills methicillin-resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion. Journal of Biophotonics, 6(9), 733-742. https://doi.org/10.1002/jbio.201200121

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title = "Antimicrobial photodynamic therapy with RLP068 kills methicillin-resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion",

abstract = "Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse skin abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process.",

keywords = "Antimicrobial photodynamic therapy, Cationic zinc phthalocyanine bioluminescence imaging, Methicillin resistant Staphylococcus aureus, Skin abrasion, Skin and soft tissue infection, Wound healing",

author = "Daniela Vecchio and Tianhong Dai and Liyi Huang and Lia Fantetti and Gabrio Roncucci and Hamblin, {Michael R.}",

year = "2013",

month = sep,

doi = "10.1002/jbio.201200121",

language = "English",

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Vecchio, D, Dai, T, Huang, L, Fantetti, L, Roncucci, G & Hamblin, MR 2013, 'Antimicrobial photodynamic therapy with RLP068 kills methicillin-resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion', Journal of Biophotonics, vol. 6, no. 9, pp. 733-742. https://doi.org/10.1002/jbio.201200121

Antimicrobial photodynamic therapy with RLP068 kills methicillin-resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion. / Vecchio, Daniela; Dai, Tianhong; Huang, Liyi et al.
In: Journal of Biophotonics, Vol. 6, No. 9, 09.2013, p. 733-742.

Research output: Contribution to journal › Article › peer-review

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AU - Dai, Tianhong

AU - Huang, Liyi

AU - Fantetti, Lia

AU - Roncucci, Gabrio

AU - Hamblin, Michael R.

PY - 2013/9

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AB - Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse skin abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process.

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Antimicrobial photodynamic therapy with RLP068 kills methicillin-resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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