Antimicrobial photodynamic therapy with RLP068 kills methicillin-resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion

Daniela Vecchio, Tianhong Dai, Liyi Huang, Lia Fantetti, Gabrio Roncucci, Michael R. Hamblin

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)

Abstract

Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse skin abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process.

Original languageEnglish
Pages (from-to)733-742
Number of pages10
JournalJournal of Biophotonics
Volume6
Issue number9
DOIs
Publication statusPublished - Sept 2013
Externally publishedYes

Keywords

  • Antimicrobial photodynamic therapy
  • Cationic zinc phthalocyanine bioluminescence imaging
  • Methicillin resistant Staphylococcus aureus
  • Skin abrasion
  • Skin and soft tissue infection
  • Wound healing

ASJC Scopus subject areas

  • General Chemistry
  • General Materials Science
  • General Biochemistry,Genetics and Molecular Biology
  • General Engineering
  • General Physics and Astronomy

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