Antimicrobial compounds from Coleonema album (Rutaceae)

Lindy L. Esterhuizen, Riaan Meyer, Ian A. Dubery

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Coleonema album, a member of the South African fynbos biome, was evaluated for its antimicrobial activity associated with its secondary metabolites. Ethanol- and acetone-based extracts obtained from plants from two different geographical areas were analyzed. A bioassay-guided fractionation methodology was followed for rapid and effective screening for the presence of bioactive compounds. The TLC-bioautographic method, used to screen the plant extracts for antimicrobial activity and localization of the active compounds, indicated the presence of a number of inhibitory compounds with activity against the microorganisms (E. coli, B. subtilis, E. faecalis, P. aeruginosa, S. aureus, M. smegmatis, M. tuberculosis, C. albicans, C. cucumerinum) tested. Evaluation of the inhibitory strength of each extract by the serial microdilution assay indicated that the C. album extracts inhibited effectively all the microorganisms, with the minimum inhibitory concentrations in the low mg ml -1 range. Identification and structural information of the bioactive components were obtained by a combination of preparative TLC and LC-MS. It revealed the presence of coumarin aglycones which were responsible for the observed antimicrobial activities. The results of this study indicate that C. album possesses strong antimicrobial activity against a wide range of microorganisms that warrants further investigation into the use of the extracts or their active constituents as a potential source for novel drugs.

Original languageEnglish
Pages (from-to)489-498
Number of pages10
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume61
Issue number7-8
DOIs
Publication statusPublished - 2006

Keywords

  • Antimicrobial
  • Coleonema album
  • Coumarins

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology

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