Antimicrobial blue light inactivation of Pseudomonas aeruginosa by photo-excitation of endogenous porphyrins: In vitro and in vivo studies

Rehab M. Amin, Brijesh Bhayana, Michael R. Hamblin, Tianhong Dai

Research output: Contribution to journalArticlepeer-review

126 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is among the most common pathogens that cause nosocomial infections and is responsible for about 10% of all hospital-acquired infections. In the present study, we investigated the potential development of tolerance of P. aeruginosa to antimicrobial blue light by carrying 10 successive cycles of sublethal blue light inactivation. The high-performance liquid chromatographic (HPLC) analysis was performed to identify endogenous porphyrins in P. aeruginosa cells. In addition, we tested the effectiveness of antimicrobial blue light in a mouse model of nonlethal skin abrasion infection by using a bioluminescent strain of P. aeruginosa. The results demonstrated that no tolerance was developed to antimicrobial blue light in P. aeruginosa after 10 cycles of sub-lethal inactivation. HPLC analysis showed that P. aeruginosa is capable of producing endogenous porphyrins in particularly, coproporphyrin III, which are assumed to be responsible for the photodynamic effects of blue light alone. P. aeruginosa infection was eradicated by antimicrobial blue light alone (48 J/cm2) without any added photosensitizer molecules in the mouse model. In conclusion, endogenous photosensitization using blue light should gain considerable attention as an effective and safe alternative antimicrobial therapy for skin infections. Lasers Surg. Med. 48:562–568, 2016.

Original languageEnglish
Pages (from-to)562-568
Number of pages7
JournalLasers in Surgery and Medicine
Volume48
Issue number5
DOIs
Publication statusPublished - 1 Jul 2016
Externally publishedYes

Keywords

  • Pseudomonas aeruginosa
  • blue light
  • drug resistance
  • endogenous porphyrins
  • mouse

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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