Antimicrobial blue light inactivation of Candida albicans: In vitro and in vivo studies

Yunsong Zhang, Yingbo Zhu, Jia Chen, Yucheng Wang, Margaret E. Sherwood, Clinton K. Murray, Mark S. Vrahas, David C. Hooper, Michael R. Hamblin, Tianhong Dai

Research output: Contribution to journalArticlepeer-review

77 Citations (Scopus)

Abstract

ABSTRACT: Fungal infections are a common cause of morbidity, mortality and cost in critical care populations. The increasing emergence of antimicrobial resistance necessitates the development of new therapeutic approaches for fungal infections. In the present study, we investigated the effectiveness of an innovative approach, antimicrobial blue light (aBL), for inactivation of Candida albicansin vitro and in infected mouse burns. A bioluminescent strain of C. albicans was used. The susceptibilities to aBL (415 nm) were compared between C. albicans and human keratinocytes. The potential development of aBL resistance by C. albicans was investigated via 10 serial passages of C. albicans on aBL exposure. For the animal study, a mouse model of thermal burn infected with the bioluminescent C. albicans strain was used. aBL was delivered to mouse burns approximately 12 h after fungal inoculation. Bioluminescence imaging was performed to monitor in real time the extent of infection in mice. The results obtained from the studies demonstrated that C. albicans was approximately 42-fold more susceptible to aBL than human keratinocytes. Serial passaging of C. albicans on aBL exposure implied a tendency of reduced aBL susceptibility of C. albicans with increasing numbers of passages; however, no statistically significant difference was observed in the post-aBL survival rate of C. albicans between the first and the last passage (P>0.05). A single exposure of 432 J/cm2 aBL reduced the fungal burden in infected mouse burns by 1.75-log10 (P=0.015). Taken together, our findings suggest aBL is a potential therapeutic for C. albicans infections.

Original languageEnglish
Pages (from-to)536-545
Number of pages10
JournalVirulence
Volume7
Issue number5
DOIs
Publication statusPublished - 3 Jul 2016
Externally publishedYes

Keywords

  • antimicrobial blue light
  • bioluminescence imagining
  • burn
  • candida albicans
  • endogenous photosensitizer
  • mouse model

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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