TY - JOUR
T1 - Anticancer activity and metabolite profiling data of Penicillium janthinellum KTMT5
AU - Tapfuma, Kudzanai Ian
AU - Sebola, Tendani Edith
AU - Uche-Okereafor, Nkemdinma
AU - Koopman, Jody
AU - Hussan, Raeesa
AU - Makatini, Maya Mellisa
AU - Mekuto, Lukhanyo
AU - Mavumengwana, Vuyo
N1 - Publisher Copyright:
© 2019 The Author(s)
PY - 2020/2
Y1 - 2020/2
N2 - Fungi are ubiquitous, they proliferate even in environments with toxic pollutants that are otherwise harmful to other eukaryotes. This article presents data of fungi which were isolated from gold mine tailings and identified by DNA sequencing of their inter transcribed spacer regions 1 and 2. Five fungal isolates were identified, among which the crude extract of Penicillium janthinellum KTMT5 was investigated for anticancer activity on A549 (lung carcinoma) and UMG87 (glioblastoma) cell lines. Untargeted metabolite profiling of the crude extract of P. janthinellum KTMT5 was performed using liquid chromatography quadrupole time of flight tandem mass spectrometry (LC-QTOF-MS/MS) and a molecular network generated using the online workflow on the Global Natural Product Social molecular networking (GNPS) website. DNA sequencing showed that all fungal isolates belonged to phylum Ascomycota with the genus Penicillium representing 75% of the fungal isolates. P. janthinellum KTMT5 which was selected for further experiments showed significant anticancer activity against UMG87 cells with a calculated IC50 value of 44.23 μg/mL in the MTS assay, while the real time xCELLigence assay showed dose-dependent anticancer activity at 50 and 100 μg/mL. Metabolite profiling revealed the presence of several known metabolites in the crude extract of P. janthinellum KTMT5 and molecular networking showed the relationships among these metabolites.
AB - Fungi are ubiquitous, they proliferate even in environments with toxic pollutants that are otherwise harmful to other eukaryotes. This article presents data of fungi which were isolated from gold mine tailings and identified by DNA sequencing of their inter transcribed spacer regions 1 and 2. Five fungal isolates were identified, among which the crude extract of Penicillium janthinellum KTMT5 was investigated for anticancer activity on A549 (lung carcinoma) and UMG87 (glioblastoma) cell lines. Untargeted metabolite profiling of the crude extract of P. janthinellum KTMT5 was performed using liquid chromatography quadrupole time of flight tandem mass spectrometry (LC-QTOF-MS/MS) and a molecular network generated using the online workflow on the Global Natural Product Social molecular networking (GNPS) website. DNA sequencing showed that all fungal isolates belonged to phylum Ascomycota with the genus Penicillium representing 75% of the fungal isolates. P. janthinellum KTMT5 which was selected for further experiments showed significant anticancer activity against UMG87 cells with a calculated IC50 value of 44.23 μg/mL in the MTS assay, while the real time xCELLigence assay showed dose-dependent anticancer activity at 50 and 100 μg/mL. Metabolite profiling revealed the presence of several known metabolites in the crude extract of P. janthinellum KTMT5 and molecular networking showed the relationships among these metabolites.
KW - Anticancer
KW - LC-QTOF-MS/MS
KW - Metabolite profiling
KW - Mine tailings
KW - Penicillium janthinellum
UR - http://www.scopus.com/inward/record.url?scp=85076438339&partnerID=8YFLogxK
U2 - 10.1016/j.dib.2019.104959
DO - 10.1016/j.dib.2019.104959
M3 - Article
AN - SCOPUS:85076438339
SN - 2352-3409
VL - 28
JO - Data in Brief
JF - Data in Brief
M1 - 104959
ER -
