Anti-diabetic effect of the ethyl acetate fraction of Clerodendrum volubile: protocatechuic acid suppresses phagocytic oxidative burst and modulates inflammatory cytokines

Ochuko L. Erukainure; M. Iqbal Choudhary; Achyut Adhikari; Md Shahidul Islam; Rahman M. Hafizur; Ahmed M. Mesaik; Aliyu Muhammad; Olubunmi Atolani; Priyanka Banerjee; Robert Preissner

doi:10.1016/j.biopha.2016.12.035

Anti-diabetic effect of the ethyl acetate fraction of Clerodendrum volubile: protocatechuic acid suppresses phagocytic oxidative burst and modulates inflammatory cytokines

Ochuko L. Erukainure
, M. Iqbal Choudhary
, Achyut Adhikari
, Md Shahidul Islam
, Rahman M. Hafizur
, Ahmed M. Mesaik
, Aliyu Muhammad
, Olubunmi Atolani
, Priyanka Banerjee
, Robert Preissner

Federal Institute of Industrial Research
University of Karachi
University of KwaZulu-Natal
University of Tabuk
Ahmadu Bello University
Charité – Universitätsmedizin Berlin
University of Ilorin

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

The antidiabetic effects of the ethyl acetate (EtOAc) fraction of Clerodendrum volubile leaves was investigated in this study. EtOAc was also fractionated to isolate the active compounds. The structure of the isolated compound (Protocatechuic acid) was established using ¹H and ¹³C NMR spectroscopies and mass spectrometry. Protocatechuic acid was investigated for its anti-oxidative burst in polymorphonuclear neutrophils (PMNs) and macrophages. It was also docked with α–glucosidase and TNF-α. Acute treatment with EtOAc fraction of Clerodendrum volubile leaves significantly (p < 0.05) decreased blood glucose level and hepatic biomarkers, and significantly (p < 0.05) increased serum insulin level and β–cell function. It had little or no effect on serum lipid profile and atherogenic indices. Protocatechuic acid significantly (p < 0.05) suppressed phagocytic oxidative burst and docked well with α–glucosidase and TNF-α. These results indicate the therapeutic effect of EtOAc fraction of C. volubile on type 2 diabetes and its complications, which can be attributed to the main bioactive compound, protocatechuic acid.

Original language	English
Pages (from-to)	307-315
Number of pages	9
Journal	Biomedicine and Pharmacotherapy
Volume	86
DOIs	https://doi.org/10.1016/j.biopha.2016.12.035
Publication status	Published - 1 Feb 2017
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Clerodendrum volubile
Oxidative burst
Protocatechuic acid
Type 2 diabetes
α–Glucosidase

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/j.biopha.2016.12.035

Cite this

Erukainure, O. L., Choudhary, M. I., Adhikari, A., Islam, M. S., Hafizur, R. M., Mesaik, A. M., Muhammad, A., Atolani, O., Banerjee, P., & Preissner, R. (2017). Anti-diabetic effect of the ethyl acetate fraction of Clerodendrum volubile: protocatechuic acid suppresses phagocytic oxidative burst and modulates inflammatory cytokines. Biomedicine and Pharmacotherapy, 86, 307-315. https://doi.org/10.1016/j.biopha.2016.12.035

@article{456a136b010b43e896bb9990e249964b,

title = "Anti-diabetic effect of the ethyl acetate fraction of Clerodendrum volubile: protocatechuic acid suppresses phagocytic oxidative burst and modulates inflammatory cytokines",

abstract = "The antidiabetic effects of the ethyl acetate (EtOAc) fraction of Clerodendrum volubile leaves was investigated in this study. EtOAc was also fractionated to isolate the active compounds. The structure of the isolated compound (Protocatechuic acid) was established using 1H and 13C NMR spectroscopies and mass spectrometry. Protocatechuic acid was investigated for its anti-oxidative burst in polymorphonuclear neutrophils (PMNs) and macrophages. It was also docked with α–glucosidase and TNF-α. Acute treatment with EtOAc fraction of Clerodendrum volubile leaves significantly (p < 0.05) decreased blood glucose level and hepatic biomarkers, and significantly (p < 0.05) increased serum insulin level and β–cell function. It had little or no effect on serum lipid profile and atherogenic indices. Protocatechuic acid significantly (p < 0.05) suppressed phagocytic oxidative burst and docked well with α–glucosidase and TNF-α. These results indicate the therapeutic effect of EtOAc fraction of C. volubile on type 2 diabetes and its complications, which can be attributed to the main bioactive compound, protocatechuic acid.",

keywords = "Clerodendrum volubile, Oxidative burst, Protocatechuic acid, Type 2 diabetes, α–Glucosidase",

author = "Erukainure, \{Ochuko L.\} and Choudhary, \{M. Iqbal\} and Achyut Adhikari and Islam, \{Md Shahidul\} and Hafizur, \{Rahman M.\} and Mesaik, \{Ahmed M.\} and Aliyu Muhammad and Olubunmi Atolani and Priyanka Banerjee and Robert Preissner",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Masson SAS",

year = "2017",

month = feb,

day = "1",

doi = "10.1016/j.biopha.2016.12.035",

language = "English",

volume = "86",

pages = "307--315",

journal = "Biomedicine and Pharmacotherapy",

issn = "0753-3322",

publisher = "Elsevier Masson s.r.l.",

}

Erukainure, OL, Choudhary, MI, Adhikari, A, Islam, MS, Hafizur, RM, Mesaik, AM, Muhammad, A, Atolani, O, Banerjee, P & Preissner, R 2017, 'Anti-diabetic effect of the ethyl acetate fraction of Clerodendrum volubile: protocatechuic acid suppresses phagocytic oxidative burst and modulates inflammatory cytokines', Biomedicine and Pharmacotherapy, vol. 86, pp. 307-315. https://doi.org/10.1016/j.biopha.2016.12.035

Anti-diabetic effect of the ethyl acetate fraction of Clerodendrum volubile: protocatechuic acid suppresses phagocytic oxidative burst and modulates inflammatory cytokines. / Erukainure, Ochuko L.; Choudhary, M. Iqbal; Adhikari, Achyut et al.
In: Biomedicine and Pharmacotherapy, Vol. 86, 01.02.2017, p. 307-315.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Anti-diabetic effect of the ethyl acetate fraction of Clerodendrum volubile

T2 - protocatechuic acid suppresses phagocytic oxidative burst and modulates inflammatory cytokines

AU - Erukainure, Ochuko L.

AU - Choudhary, M. Iqbal

AU - Adhikari, Achyut

AU - Islam, Md Shahidul

AU - Hafizur, Rahman M.

AU - Mesaik, Ahmed M.

AU - Muhammad, Aliyu

AU - Atolani, Olubunmi

AU - Banerjee, Priyanka

AU - Preissner, Robert

PY - 2017/2/1

Y1 - 2017/2/1

N2 - The antidiabetic effects of the ethyl acetate (EtOAc) fraction of Clerodendrum volubile leaves was investigated in this study. EtOAc was also fractionated to isolate the active compounds. The structure of the isolated compound (Protocatechuic acid) was established using 1H and 13C NMR spectroscopies and mass spectrometry. Protocatechuic acid was investigated for its anti-oxidative burst in polymorphonuclear neutrophils (PMNs) and macrophages. It was also docked with α–glucosidase and TNF-α. Acute treatment with EtOAc fraction of Clerodendrum volubile leaves significantly (p < 0.05) decreased blood glucose level and hepatic biomarkers, and significantly (p < 0.05) increased serum insulin level and β–cell function. It had little or no effect on serum lipid profile and atherogenic indices. Protocatechuic acid significantly (p < 0.05) suppressed phagocytic oxidative burst and docked well with α–glucosidase and TNF-α. These results indicate the therapeutic effect of EtOAc fraction of C. volubile on type 2 diabetes and its complications, which can be attributed to the main bioactive compound, protocatechuic acid.

AB - The antidiabetic effects of the ethyl acetate (EtOAc) fraction of Clerodendrum volubile leaves was investigated in this study. EtOAc was also fractionated to isolate the active compounds. The structure of the isolated compound (Protocatechuic acid) was established using 1H and 13C NMR spectroscopies and mass spectrometry. Protocatechuic acid was investigated for its anti-oxidative burst in polymorphonuclear neutrophils (PMNs) and macrophages. It was also docked with α–glucosidase and TNF-α. Acute treatment with EtOAc fraction of Clerodendrum volubile leaves significantly (p < 0.05) decreased blood glucose level and hepatic biomarkers, and significantly (p < 0.05) increased serum insulin level and β–cell function. It had little or no effect on serum lipid profile and atherogenic indices. Protocatechuic acid significantly (p < 0.05) suppressed phagocytic oxidative burst and docked well with α–glucosidase and TNF-α. These results indicate the therapeutic effect of EtOAc fraction of C. volubile on type 2 diabetes and its complications, which can be attributed to the main bioactive compound, protocatechuic acid.

KW - Clerodendrum volubile

KW - Oxidative burst

KW - Protocatechuic acid

KW - Type 2 diabetes

KW - α–Glucosidase

UR - https://www.scopus.com/pages/publications/85006968177

U2 - 10.1016/j.biopha.2016.12.035

DO - 10.1016/j.biopha.2016.12.035

M3 - Article

C2 - 28011378

AN - SCOPUS:85006968177

SN - 0753-3322

VL - 86

SP - 307

EP - 315

JO - Biomedicine and Pharmacotherapy

JF - Biomedicine and Pharmacotherapy

ER -

Anti-diabetic effect of the ethyl acetate fraction of Clerodendrum volubile: protocatechuic acid suppresses phagocytic oxidative burst and modulates inflammatory cytokines

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this