Animal models and preclinical tests to evaluate in situ-forming hydrogels

In situ-forming hydrogels have emerged as versatile biomaterials with significant potential for various biomedical applications, including drug delivery, tissue engineering, and wound healing. Their ability to undergo a phase transition from a liquid to a gel state within the body offers distinct advantages in localized and sustained drug release, as well as tissue regeneration. However, before these hydrogels can be translated into clinical practice, rigorous preclinical evaluation is essential. This chapter provides an overview of the current methodologies and considerations for conducting animal studies and preclinical tests to assess the safety, efficacy, and performance of in situ-forming hydrogels. In conclusion, this book chapter offers a comprehensive overview of the essential considerations and methodologies for conducting preclinical evaluations of in situ-forming hydrogels. By providing insights into the selection of appropriate animal models, ethical considerations, and a wide array of preclinical tests, it aims to guide researchers and practitioners in assessing the safety and efficacy of these promising biomaterials, ultimately advancing their translation from bench to bedside.