An inexpensive, automated instrument for laser irradiation of cultured cells

Objective: Laser irradiation of cultured cells is a valuable technique for elucidating the mechanisms of low-level laser therapy, but is often tedious because of the need to manually change the position of the laser beam. Consequently, we developed a computer-based system that automatically moves a cell culture plate over a laser beam and times the exposure. Background Data: There are presently no commercial devices available for automated laser irradiation of cultured cells. Many investigators thus manually aim and time laser exposure, a time-consuming task that is prone to errors. Materials and Methods: We used outdated, surplus computer components to construct a system for automated laser exposure of cultured cells. This design strategy makes the system quite inexpensive. Results: Construction and operation of the system is described and an example of its use is presented. Alternate means of accomplishing automated laser irradiation are also presented. Conclusion: Inexpensive and relatively simple devices can be constructed for automated laser irradiation of cultured cells. These devices can eliminate the tedium and errors of manual laser exposure.