An improved method for the purification of the nucleic acids from small amounts of Escherichia coli

Allen C. Rogerson

doi:10.1016/0003-2697(75)90487-X

An improved method for the purification of the nucleic acids from small amounts of Escherichia coli

Allen C. Rogerson

Bryn Mawr College

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

An improved method for the rapid extraction and purification of the nucleic acids from small amounts of Escherichia coli is described. The method is applicable to multiple samples of about 5 × 10⁸ cells each, and can be completed in about 4-6 hr. Recovery is on the order of 60%, there is little detectable degradation or selective loss of RNA, and virtually all of the cellular-derived carbon in the final preparation is nucleic acid.

Original language	English
Pages (from-to)	34-41
Number of pages	8
Journal	Analytical Biochemistry
Volume	65
Issue number	1-2
DOIs	https://doi.org/10.1016/0003-2697(75)90487-X
Publication status	Published - 12 May 1975
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(75)90487-X

Cite this

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abstract = "An improved method for the rapid extraction and purification of the nucleic acids from small amounts of Escherichia coli is described. The method is applicable to multiple samples of about 5 × 108 cells each, and can be completed in about 4-6 hr. Recovery is on the order of 60%, there is little detectable degradation or selective loss of RNA, and virtually all of the cellular-derived carbon in the final preparation is nucleic acid.",

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N2 - An improved method for the rapid extraction and purification of the nucleic acids from small amounts of Escherichia coli is described. The method is applicable to multiple samples of about 5 × 108 cells each, and can be completed in about 4-6 hr. Recovery is on the order of 60%, there is little detectable degradation or selective loss of RNA, and virtually all of the cellular-derived carbon in the final preparation is nucleic acid.

AB - An improved method for the rapid extraction and purification of the nucleic acids from small amounts of Escherichia coli is described. The method is applicable to multiple samples of about 5 × 108 cells each, and can be completed in about 4-6 hr. Recovery is on the order of 60%, there is little detectable degradation or selective loss of RNA, and virtually all of the cellular-derived carbon in the final preparation is nucleic acid.

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DO - 10.1016/0003-2697(75)90487-X

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JO - Analytical Biochemistry

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ER -

An improved method for the purification of the nucleic acids from small amounts of Escherichia coli

Abstract

ASJC Scopus subject areas

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