An electrochemical DNA biosensor developed on novel multinuclear nickel(II) salicylaldimine metallodendrimer platform

Omotayo A. Arotiba; Anna Ignaszak; Rehana Malgas; Amir Al-Ahmed; Priscilla G.L. Baker; Selwyn F. Mapolie; Emmanuel I. Iwuoha

doi:10.1016/j.electacta.2007.08.016

An electrochemical DNA biosensor developed on novel multinuclear nickel(II) salicylaldimine metallodendrimer platform

Omotayo A. Arotiba, Anna Ignaszak, Rehana Malgas, Amir Al-Ahmed, Priscilla G.L. Baker, Selwyn F. Mapolie, Emmanuel I. Iwuoha

University of the Western Cape

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

An electrochemical DNA biosensor (EDB) was prepared using an oligonucleotide of 21 bases with sequence NH₂-5′-GAGGAGTTGGGGGAGCACATT-3′ (probe DNA) immobilized on a novel multinuclear nickel(II) salicylaldimine metallodendrimer on glassy carbon electrode (GCE). The metallodendrimer was synthesized from amino functionalized polypropylene imine dendrimer, DAB-(NH₂)₈. The EDB was prepared by depositing probe DNA on a dendrimer-modified GCE surface and left to immobilize for 1 h. Voltammetric and electrochemical impedance spectroscopic (EIS) studies were carried out to characterize the novel metallodendrimer, the EDB and its hybridization response in PBS using [Fe(CN)₆]^3-/4- as a redox probe at pH 7.2. The metallodendrimer was electroactive in PBS with two reversible redox couples at E°′ = +200 mV and E°′ = +434 mV; catalytic by reducing the E_pa of [Fe(CN)₆]^3-/4- by 22 mV; conducting and has diffusion coefficient of 8.597 × 10^-8 cm² s^-1. From the EIS circuit fitting results, the EDB responded to 5 nM target DNA by exhibiting a decrease in charge transfer resistance (R_ct) in PBS and increase in R_ct in [Fe(CN)₆]^3-/4- redox probe; while in voltammetry, increase in peak anodic current was observed in PBS after hybridization, thus giving the EDB a dual probe advantage.

Original language	English
Pages (from-to)	1689-1696
Number of pages	8
Journal	Electrochimica Acta
Volume	53
Issue number	4
DOIs	https://doi.org/10.1016/j.electacta.2007.08.016
Publication status	Published - 31 Dec 2007
Externally published	Yes

Keywords

Dendrimer
Electrochemical DNA biosensor
Electrochemical impedance spectroscopy
Immobilization
Metallodendrimer

ASJC Scopus subject areas

General Chemical Engineering
Electrochemistry

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10.1016/j.electacta.2007.08.016

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@article{c2a070e277c941be984d49a08a2bd49f,

title = "An electrochemical DNA biosensor developed on novel multinuclear nickel(II) salicylaldimine metallodendrimer platform",

abstract = "An electrochemical DNA biosensor (EDB) was prepared using an oligonucleotide of 21 bases with sequence NH2-5′-GAGGAGTTGGGGGAGCACATT-3′ (probe DNA) immobilized on a novel multinuclear nickel(II) salicylaldimine metallodendrimer on glassy carbon electrode (GCE). The metallodendrimer was synthesized from amino functionalized polypropylene imine dendrimer, DAB-(NH2)8. The EDB was prepared by depositing probe DNA on a dendrimer-modified GCE surface and left to immobilize for 1 h. Voltammetric and electrochemical impedance spectroscopic (EIS) studies were carried out to characterize the novel metallodendrimer, the EDB and its hybridization response in PBS using [Fe(CN)6]3-/4- as a redox probe at pH 7.2. The metallodendrimer was electroactive in PBS with two reversible redox couples at E°′ = +200 mV and E°′ = +434 mV; catalytic by reducing the Epa of [Fe(CN)6]3-/4- by 22 mV; conducting and has diffusion coefficient of 8.597 × 10-8 cm2 s-1. From the EIS circuit fitting results, the EDB responded to 5 nM target DNA by exhibiting a decrease in charge transfer resistance (Rct) in PBS and increase in Rct in [Fe(CN)6]3-/4- redox probe; while in voltammetry, increase in peak anodic current was observed in PBS after hybridization, thus giving the EDB a dual probe advantage.",

keywords = "Dendrimer, Electrochemical DNA biosensor, Electrochemical impedance spectroscopy, Immobilization, Metallodendrimer",

author = "Arotiba, {Omotayo A.} and Anna Ignaszak and Rehana Malgas and Amir Al-Ahmed and Baker, {Priscilla G.L.} and Mapolie, {Selwyn F.} and Iwuoha, {Emmanuel I.}",

year = "2007",

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language = "English",

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T1 - An electrochemical DNA biosensor developed on novel multinuclear nickel(II) salicylaldimine metallodendrimer platform

AU - Arotiba, Omotayo A.

AU - Ignaszak, Anna

AU - Malgas, Rehana

AU - Al-Ahmed, Amir

AU - Baker, Priscilla G.L.

AU - Mapolie, Selwyn F.

AU - Iwuoha, Emmanuel I.

PY - 2007/12/31

Y1 - 2007/12/31

N2 - An electrochemical DNA biosensor (EDB) was prepared using an oligonucleotide of 21 bases with sequence NH2-5′-GAGGAGTTGGGGGAGCACATT-3′ (probe DNA) immobilized on a novel multinuclear nickel(II) salicylaldimine metallodendrimer on glassy carbon electrode (GCE). The metallodendrimer was synthesized from amino functionalized polypropylene imine dendrimer, DAB-(NH2)8. The EDB was prepared by depositing probe DNA on a dendrimer-modified GCE surface and left to immobilize for 1 h. Voltammetric and electrochemical impedance spectroscopic (EIS) studies were carried out to characterize the novel metallodendrimer, the EDB and its hybridization response in PBS using [Fe(CN)6]3-/4- as a redox probe at pH 7.2. The metallodendrimer was electroactive in PBS with two reversible redox couples at E°′ = +200 mV and E°′ = +434 mV; catalytic by reducing the Epa of [Fe(CN)6]3-/4- by 22 mV; conducting and has diffusion coefficient of 8.597 × 10-8 cm2 s-1. From the EIS circuit fitting results, the EDB responded to 5 nM target DNA by exhibiting a decrease in charge transfer resistance (Rct) in PBS and increase in Rct in [Fe(CN)6]3-/4- redox probe; while in voltammetry, increase in peak anodic current was observed in PBS after hybridization, thus giving the EDB a dual probe advantage.

AB - An electrochemical DNA biosensor (EDB) was prepared using an oligonucleotide of 21 bases with sequence NH2-5′-GAGGAGTTGGGGGAGCACATT-3′ (probe DNA) immobilized on a novel multinuclear nickel(II) salicylaldimine metallodendrimer on glassy carbon electrode (GCE). The metallodendrimer was synthesized from amino functionalized polypropylene imine dendrimer, DAB-(NH2)8. The EDB was prepared by depositing probe DNA on a dendrimer-modified GCE surface and left to immobilize for 1 h. Voltammetric and electrochemical impedance spectroscopic (EIS) studies were carried out to characterize the novel metallodendrimer, the EDB and its hybridization response in PBS using [Fe(CN)6]3-/4- as a redox probe at pH 7.2. The metallodendrimer was electroactive in PBS with two reversible redox couples at E°′ = +200 mV and E°′ = +434 mV; catalytic by reducing the Epa of [Fe(CN)6]3-/4- by 22 mV; conducting and has diffusion coefficient of 8.597 × 10-8 cm2 s-1. From the EIS circuit fitting results, the EDB responded to 5 nM target DNA by exhibiting a decrease in charge transfer resistance (Rct) in PBS and increase in Rct in [Fe(CN)6]3-/4- redox probe; while in voltammetry, increase in peak anodic current was observed in PBS after hybridization, thus giving the EDB a dual probe advantage.

KW - Dendrimer

KW - Electrochemical DNA biosensor

KW - Electrochemical impedance spectroscopy

KW - Immobilization

KW - Metallodendrimer

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VL - 53

SP - 1689

EP - 1696

JO - Electrochimica Acta

JF - Electrochimica Acta

IS - 4

ER -

An electrochemical DNA biosensor developed on novel multinuclear nickel(II) salicylaldimine metallodendrimer platform

Abstract

Keywords

ASJC Scopus subject areas

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