An Electrochemical Aptamer Biosensor for Bisphenol A on a Carbon Nanofibre-silver Nanoparticle Immobilisation Platform

Tebogo R. Tsekeli; Luthando Tshwenya; Tsholofelo I. Sebokolodi; Thabile Ndlovu; Omotayo A. Arotiba

doi:10.1002/elan.202100167

An Electrochemical Aptamer Biosensor for Bisphenol A on a Carbon Nanofibre-silver Nanoparticle Immobilisation Platform

Tebogo R. Tsekeli, Luthando Tshwenya, Tsholofelo I. Sebokolodi, Thabile Ndlovu, Omotayo A. Arotiba

Chemical Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A nanocomposite platform of silver nanoparticles and carbon nanofibres (AgCNFs) was used to immobilise a bisphenol A specific 63-mer ssDNA aptamer to form a biosensor. The fabrication process of the biosensor was studied with electrochemical impedance spectroscopy and cyclic voltammetry in the presence of [Fe(CN)₆]^3−/4− as redox probe. The biosensor detected bisphenol A in a linear range of 0.1–10 nM, with a limit of detection of 0.39 nM using square wave voltammetry (SWV). The biosensor exhibited good selectivity in the presence of interfering species at 100-fold concentrations and was used to detect BPA in real water sample.

Original language	English
Pages (from-to)	2053-2061
Number of pages	9
Journal	Electroanalysis
Volume	33
Issue number	9
DOIs	https://doi.org/10.1002/elan.202100167
Publication status	Published - Sept 2021

Keywords

Bisphenol A
aptamer
biosensor
carbon nanofibres
immobilisation platform
silver nanoparticles

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

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10.1002/elan.202100167

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title = "An Electrochemical Aptamer Biosensor for Bisphenol A on a Carbon Nanofibre-silver Nanoparticle Immobilisation Platform",

abstract = "A nanocomposite platform of silver nanoparticles and carbon nanofibres (AgCNFs) was used to immobilise a bisphenol A specific 63-mer ssDNA aptamer to form a biosensor. The fabrication process of the biosensor was studied with electrochemical impedance spectroscopy and cyclic voltammetry in the presence of [Fe(CN)6]3−/4− as redox probe. The biosensor detected bisphenol A in a linear range of 0.1–10 nM, with a limit of detection of 0.39 nM using square wave voltammetry (SWV). The biosensor exhibited good selectivity in the presence of interfering species at 100-fold concentrations and was used to detect BPA in real water sample.",

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AU - Tsekeli, Tebogo R.

AU - Tshwenya, Luthando

AU - Sebokolodi, Tsholofelo I.

AU - Ndlovu, Thabile

AU - Arotiba, Omotayo A.

PY - 2021/9

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AB - A nanocomposite platform of silver nanoparticles and carbon nanofibres (AgCNFs) was used to immobilise a bisphenol A specific 63-mer ssDNA aptamer to form a biosensor. The fabrication process of the biosensor was studied with electrochemical impedance spectroscopy and cyclic voltammetry in the presence of [Fe(CN)6]3−/4− as redox probe. The biosensor detected bisphenol A in a linear range of 0.1–10 nM, with a limit of detection of 0.39 nM using square wave voltammetry (SWV). The biosensor exhibited good selectivity in the presence of interfering species at 100-fold concentrations and was used to detect BPA in real water sample.

KW - Bisphenol A

KW - aptamer

KW - biosensor

KW - carbon nanofibres

KW - immobilisation platform

KW - silver nanoparticles

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An Electrochemical Aptamer Biosensor for Bisphenol A on a Carbon Nanofibre-silver Nanoparticle Immobilisation Platform

Abstract

Keywords

ASJC Scopus subject areas

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