Aflatoxins: Occurrence, biosynthesis, mechanism of action and effects, conventional/emerging detection techniques

Aflatoxins (AFs) are toxic secondary metabolites prevalent in various food and agricultural products, posing significant challenges to global food safety. The detection and quantification of AFs through high-precision analytical techniques are crucial in mitigating AF contamination levels and associated health risks. Various methods, including conventional and emerging techniques, have been developed for detecting and quantifying AFs in food samples. This review provides an in-depth analysis of the global occurrence of AF in food commodities, covering their biosynthesis, mode of action, and effects on humans and animals. Additionally, the review discusses different conventional strategies, including chromatographic and immunochemical approaches, for AF quantification and identification in food samples. Furthermore, emerging AF detection strategies, such as solid-state gas sensors and electronic nose technologies, along with their applications, limitations, and future perspectives, were reviewed. Sample purification, along with their respective advantages and limitations, are also discussed herein.