TY - JOUR
T1 - Advances in detection of fastidious bacteria
T2 - From microscopic observation to molecular biosensors
AU - Mobed, Ahmad
AU - Baradaran, Behzad
AU - Guardia, Miguel de la
AU - Agazadeh, Mohammad
AU - Hasanzadeh, Mohammad
AU - Rezaee, Mohammad Ahangarzadeh
AU - Mosafer, Jafar
AU - Mokhtarzadeh, Ahad
AU - Hamblin, Michael R.
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/4
Y1 - 2019/4
N2 - Identification of pathogens and diagnosis of infections are important health challenges, especially in the case of fastidious bacteria which are those difficult-to-grow. A fastidious organism is any organism that has a complex nutritional requirement. Additionally, a fastidious microorganism will only grow when specific nutrients are included in the culture medium. These bacteria can cause serious diseases whose detection and monitoring is critical in many cases. The oldest detection methods are based on simple microscopy observation and staining, after culture on selective growth media, but often do not provide a clear answer. Some new molecular approaches, such as DNA-based sequencing and antibody-antigen binding, are particularly promising in the case of fastidious bacteria. The objective of this review is to summarize the advantages of the use of biosensors as a modern diagnostic tool in microbiology for detecting fastidious bacteria.
AB - Identification of pathogens and diagnosis of infections are important health challenges, especially in the case of fastidious bacteria which are those difficult-to-grow. A fastidious organism is any organism that has a complex nutritional requirement. Additionally, a fastidious microorganism will only grow when specific nutrients are included in the culture medium. These bacteria can cause serious diseases whose detection and monitoring is critical in many cases. The oldest detection methods are based on simple microscopy observation and staining, after culture on selective growth media, but often do not provide a clear answer. Some new molecular approaches, such as DNA-based sequencing and antibody-antigen binding, are particularly promising in the case of fastidious bacteria. The objective of this review is to summarize the advantages of the use of biosensors as a modern diagnostic tool in microbiology for detecting fastidious bacteria.
KW - Biosensors
KW - DNA based sensors
KW - Fastidious bacteria
KW - Immunosensors
KW - Traditional methods
UR - http://www.scopus.com/inward/record.url?scp=85061829682&partnerID=8YFLogxK
U2 - 10.1016/j.trac.2019.02.012
DO - 10.1016/j.trac.2019.02.012
M3 - Review article
AN - SCOPUS:85061829682
SN - 0165-9936
VL - 113
SP - 157
EP - 171
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
ER -