A Snapshot of the Influent and Effluent Bacterial Populations in a Wastewater Treatment Plant in the North-West Province, South Africa

Stenly Makuwa, Ezekiel Green, Elvis Fosso-Kankeu, Victor Moroaswi, Matsobane Tlou

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Wastewater treatment plants receive influent wastewater that is contaminated with bacterial pathogens which may be released into the environment if the plant effluent is inadequately treated. In this study, next-generation sequencing was used to perform a 16S rDNA-based survey of bacterial populations in the influent and effluent from a treatment facility in the North-West Province (SA). In total, 3638 and 3872 effective DNA reads were obtained for the influent and effluent, respectively. Sequence analysis revealed the detection of a diverse bacterial constituency in both the influent and effluent samples. The phyla: Proteobacteria (49.82% and 52.04%), Firmicutes (14.06% and 13.14%) and Actinobacteria (5.00% and 9.99%) were found to be taxonomically abundant in the influent and effluent, respectively. This translated to the detection of biological treatment-, fecal coliform-, and disease-associated bacterial groups that are classified under the following genera: Escherichia spp., Serratia spp., Aeromonas spp., Legionella spp., Pseudomonas spp., Mycobacterium spp., Clostridium spp., Staphylococcus spp. and Streptococcus spp., Comamonas spp., Nitrosomonas spp., Acinetobacter spp., Rhodobacter spp., Paracoccus spp., Hyphomicrobium spp., and Desulfovibrio spp.

Original languageEnglish
Pages (from-to)764-773
Number of pages10
JournalApplied Microbiology
Volume3
Issue number3
DOIs
Publication statusPublished - Sept 2023

Keywords

  • 16S rDNA
  • class
  • effluent
  • genera
  • influent
  • next-generation sequencing
  • phyla
  • wastewater

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Chemical Engineering (miscellaneous)
  • Applied Microbiology and Biotechnology

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