A polyamidoamine dendrimer-streptavidin supramolecular architecture for biosensor development

N. Soda; O. A. Arotiba

doi:10.1016/j.bioelechem.2017.06.012

A polyamidoamine dendrimer-streptavidin supramolecular architecture for biosensor development

N. Soda, O. A. Arotiba

Chemical Sciences

University of Johannesburg

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A novel polyamidoamine dendrimer-streptavidin supramolecular architecture suitable as a versatile platform for biosensor development is reported. The dendrimer was electrodeposited on a glassy carbon electrode via cyclic voltammetry. The dendrimer electrode was further modified with streptavidin by electrostatic attraction upon drop coating. The platform i.e. the dendrimer-streptavidin modified electrode was electrochemically interrogated in phosphate buffer, ferrocyanide and H₂O₂. The dendrimer-streptavidin platform was used in the preparation of a simple DNA biosensor as a proof of concept. The supramolecular architecture of dendrimer-streptavidin was stable, electroactive and thus lends itself as a versatile immobilisation layer for any biotinylated bioreceptors in biosensor development.

Original language	English
Pages (from-to)	14-18
Number of pages	5
Journal	Bioelectrochemistry
Volume	118
DOIs	https://doi.org/10.1016/j.bioelechem.2017.06.012
Publication status	Published - Dec 2017

Keywords

Biosensor
Electrodeposition
Polyamidoamine dendrimer
Streptavidin
Supramolecular

ASJC Scopus subject areas

Biophysics
Physical and Theoretical Chemistry
Electrochemistry

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10.1016/j.bioelechem.2017.06.012

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title = "A polyamidoamine dendrimer-streptavidin supramolecular architecture for biosensor development",

abstract = "A novel polyamidoamine dendrimer-streptavidin supramolecular architecture suitable as a versatile platform for biosensor development is reported. The dendrimer was electrodeposited on a glassy carbon electrode via cyclic voltammetry. The dendrimer electrode was further modified with streptavidin by electrostatic attraction upon drop coating. The platform i.e. the dendrimer-streptavidin modified electrode was electrochemically interrogated in phosphate buffer, ferrocyanide and H2O2. The dendrimer-streptavidin platform was used in the preparation of a simple DNA biosensor as a proof of concept. The supramolecular architecture of dendrimer-streptavidin was stable, electroactive and thus lends itself as a versatile immobilisation layer for any biotinylated bioreceptors in biosensor development.",

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AU - Soda, N.

AU - Arotiba, O. A.

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AB - A novel polyamidoamine dendrimer-streptavidin supramolecular architecture suitable as a versatile platform for biosensor development is reported. The dendrimer was electrodeposited on a glassy carbon electrode via cyclic voltammetry. The dendrimer electrode was further modified with streptavidin by electrostatic attraction upon drop coating. The platform i.e. the dendrimer-streptavidin modified electrode was electrochemically interrogated in phosphate buffer, ferrocyanide and H2O2. The dendrimer-streptavidin platform was used in the preparation of a simple DNA biosensor as a proof of concept. The supramolecular architecture of dendrimer-streptavidin was stable, electroactive and thus lends itself as a versatile immobilisation layer for any biotinylated bioreceptors in biosensor development.

KW - Biosensor

KW - Electrodeposition

KW - Polyamidoamine dendrimer

KW - Streptavidin

KW - Supramolecular

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A polyamidoamine dendrimer-streptavidin supramolecular architecture for biosensor development

Abstract

Keywords

ASJC Scopus subject areas

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