A designed peptide-based vaccine to combat Brucella melitensis, B. suis and B. abortus: Harnessing an epitope mapping and immunoinformatics approach

Hossein Tarrahimofrad, Javad Zamani, Michael R. Hamblin, Maryam Darvish, Hamed Mirzaei

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Vaccines against Brucella abortus, B. melitensis and B. suis have been based on weakened or killed bacteria, however there is no recombinant vaccine for disease prevention or therapy. This study attempted to predict IFN-γ epitopes, T cell cytotoxicity, and T lymphocytes in order to produce a multiepitope vaccine based on BtpA, Omp16, Omp28, virB10, Omp25, and Omp31 antigens against B. melitensis, B. abortus, and B. suis. AAY, GPGPG, and EAAAK peptides were used as epitope linkers, while the PADRE sequence was used as a Toll-like receptor 2 (TLR2) and TLR4 agonist. The final construct included 389 amino acids, and was a soluble protein with a molecular weight of 41.3 kDa, and nonallergenic and antigenic properties. Based on molecular docking studies, molecular dynamics simulations such as Gyration, RMSF, and RMSD, as well as tertiary structure validation methods, the modeled protein had a stable structure capable of interacting with TLR2/4. As a result, this novel vaccine may stimulate immune responses in B and T cells, and could prevent infection by B. suis, B. abortus, and B. melitensis.

Original languageEnglish
Article number113557
JournalBiomedicine and Pharmacotherapy
Volume155
DOIs
Publication statusPublished - Nov 2022

Keywords

  • B. abortus
  • B. suis
  • Brucella melitensis
  • Immunoinformatics
  • Molecular dynamics simulations
  • Peptide vaccine

ASJC Scopus subject areas

  • Pharmacology

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